Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Cymbidium ringspot tombusvirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by K. Boswell. 1985.

Nomenclature

Acronym

CyRSV (CymRSV)

Strains

cymbidium and clover.

ICTV decimal code

74.0.1.0.005

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Cymbidium spp. - chlorotic ring-mottling, more intense when mixed with cymbidium mosaic virus.
• Trifolium repens - chlorotic flecking, mottling.

Transmission

Transmitted by means not involving a vector. Virus transmitted by mechanical inoculation; transmitted by contact between plants; not transmitted by seed (in Trifolium incarnatum, Phaseolus vulgaris cv. The Prince, Nicotiana clevelandii).

Geographical distribution

Spreads in the UK.

Experimental host range

Many (>9) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor, C. murale, C. quinoa - chlorotic or necrotic local lesions; not systemic.
• Emilia sagittata - chlorotic local lesions; systemic yellow spots and rings, leaf malformation and necrosis.
• Phaseolus vulgaris cv. The Prince - whitish necrotic local lesions with Cymbidium strain, semi-necrotic local lesions with clover strain; systemic chlorosis and mottling (both strains).
• Vigna unguiculata cv. Blackeye - necrotic whitish and brown local lesions; not systemic.
• Nicotiana clevelandii - diffuse chlorotic, and some brown local lesions, necrosis spreads killing leaf; systemic chlorotic and necrotic, speckling, severe puckering, stunting.
• Nicotiana glutinosa, N. tabacum cvs White Burley, Judy's Pride, Havana 425, Xanthi-nc - brown necrotic local lesions; not systemic.

Maintenance and propagation hosts

Nicotiana clevelandii - virus is best stored as freeze-dried leaves.

Assay hosts (Local lesions or Whole plants)

Chenopodium quinoa (L), Phaseolus vulgaris (L).

Susceptible host species

• Amaranthus caudatus
• Antirrhinum majus
• Arachis hypogaea
• Atriplex hortensis
• Beta vulgaris
• Capsicum frutescens
• Celosia argentea
• Chenopodium amaranticolor
• Chenopodium murale
• Chenopodium quinoa
• Cucumis sativus
• Cymbidium
• Datura stramonium
• Emilia sagittata
• Fagopyrum esculentum
• Glycine max
• Gomphrena globosa
• Helianthus annuus
• Lactuca sativa
• Lathyrus odoratus
• Lupinus angustifolius
• Lycopersicon esculentum
• Myosotis sylvatica
• Nicandra physalodes
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana debneyi
• Nicotiana glutinosa
• Nicotiana megalosiphon
• Nicotiana rustica
• Nicotiana tabacum
• Ocimum basilicum
• Petunia × hybrida
• Phaseolus vulgaris
• Phlox drummondii
• Physalis floridana
• Phytolacca americana
• Pisum sativum
• Saponaria vaccaria
• Senecio vulgaris
• Solanum nigrum
• Spinacia oleracea
• Stellaria media
• Tetragonia tetragonioides
• Torenia fournieri
• Trifolium incarnatum
• Trifolium repens
• Tropaeolum majus
• Vicia faba
• Vigna unguiculata
• Zinnia elegans

Insusceptible host species

• Abelmoschus esculentus
• Ageratum conyzoides
• Apium graveolens
• Avena sativa
• Bellis perennis
• Brassica campestris ssp. pekinensis
• Brassica campestris ssp. rapa
• Brassica oleracea var. capitata
• Catharanthus roseus
• Cheiranthus cheiri
• Chrysanthemum morifolium
• Citrus limon
• Cucumis melo
• Dactylis glomerata
• Dianthus barbatus
• Hordeum vulgare
• Hyoscyamus niger
• Ipomoea nil (cv. Scarlett o'Hara)
• Nicotiana clevelandii
• Phleum pratense
• Solanum melongena
• Solanum tuberosum
• Triticum aestivum
• Zea mays

Families containing susceptible hosts

• Amaranthaceae (3/3)
• Boraginaceae (1/1)
• Caryophyllaceae (2/3)
• Chenopodiaceae (6/6)
• Compositae (5/8)
• Cucurbitaceae (1/2)
• Labiatae (1/1)
• Leguminosae-Papilionoideae (10/10)
• Orchidaceae (1 /1)
• Phytolaccaceae (1/1)
• Polemoniaceae (1/1)
• Polygonaceae (1/1)
• Scrophulariaceae (2/2)
• Solanaceae (14/17)
• Tetragoniaceae (1/1)
• Tropaeolaceae (1/1)

Families containing insusceptible hosts

• Apocynaceae (1/1)
• Caryophyllaceae (1/3)
• Compositae (3/8)
• Convolvulaceae (1/1)
• Cruciferae (4/4)
• Cucurbitaceae (1/2)
• Gramineae (6/6)
• Malvaceae (1/1)
• Rutaceae (1 /1)
• Solanaceae (4/17)
• Umbelliferae (1/1)

Sources of host-range data

Hollings et al. (1977).

Physical and biochemical properties

Properties of particles in sap

TIP: 85-90 °C. LIV: 300 days. DEP: log₁₀ minus 5-6. Leaf sap contains many virions.

Purification method

Hollings et al. (1977).

Particle morphology

Virions isometric; not enveloped; 30 nm in diameter.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 136-139 S. Density 1.36 g cm^-3 in CsCl.

Biochemical properties

Virions contain 14-15 % nucleic acid.

Genome consists of RNA; single-stranded. Total genome size 4.733 kb. Genome unipartite; largest (or only) genome part 4.733 kb. Base composition 27.8 % G; 24.9 % A; 21.3 % C; 26.1 % U.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• D00719 Em(40)_vi:CRVDIR Gb(84)_vi:CRVDIR Cymbidium ringspot virus (CyRSV) defective interfering (DI) RNA. 3/92 499bp.
• D00720 Em(40)_vi:CRVSATR Gb(84)_vi:CRVSATR Cymbidium ringspot virus (CyRSV) satellite RNA. 3/92 621bp.
• S43932 Em(40)_un:S43932 Gb(84)_vi:S43932 (defective interfering RNA) cymbidium ringspot tombusvirus CyRSV, DI-371, Other Genetic Mate
• X15511 Em(40)_vi:CRTV1 Gb(84)_vi:CRTV1 Cymbidium ringspot tombusvirus RNA. 9/93 4,733bp. 4 sequences.

Features of proteins

Virion protein(s) one; M_r 43600.

Cytopathology

Virions found in leaves, roots, stems and flowers.

Taxonomy and relationships

Tombusvirus: Tombusviridae

Virus(es) with serologically related virions

Some tombusviruses.

Virus(es) with serologically unrelated virions

Some tombusviruses (Koenig and Gibbs, 1986).

Comments and References

References

• Dalmay, T., Rubino, L., Burgyan, J., Kollar, A. and Russo, M. (1993). Virology 194: 697.
• Grieco, F., Burgyan, J. and Russo, M. (1989). Nucl. Acids Res. 17: 6383.
• Hollings, M. and Stone, O.M. (1963). Rep. Glasshouse Crops Res. Inst. 1962, p. 90.
• Hollings, M. and Stone, O.M. (1977). CMI/AAB Descr. Pl. Viruses No. 178, 4 pp.
• Hollings, M., Stone, O.M. and Barton, R.J. (1977). Ann. appl. Biol. 85: 233.
• Koenig, R. and Gibbs, A.J. (1986). J. gen. Virol. 67: 75.
• Rubino, L., Burgyan, J., Grieco, F. and Russo, M. (1989). J. gen. Virol. 71: 1655.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.