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Descriptions and Lists from the VIDE Database

Cymbidium mosaic potexvirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by R.I.B. Francki, 1984.

Nomenclature

Synonyms

orchid mosaic virus.

Acronym

CymMV

ICTV decimal code

56.0.1.0.007

Host range and symptoms

Natural host range and symptoms

Symptoms vary seasonally.
• Cymbidium spp. - mosaic, necrosis.
• Cattleya spp. - mosaic, flower necrosis.
• Phalaenopsis spp. - mosaic, water soaked local lesions.
• Vanda spp. - chlorotic flecks.
• Epidendrum spp., Laelia spp., Laeliocattleya spp., Oncidium spp., Zygopetalum spp., Vanilla fragrans - also naturally infected.

Transmission

Transmitted by means not involving a vector. Virus transmitted by mechanical inoculation; transmitted by contact between plants.

Geographical distribution

Probably distributed worldwide.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor, Datura stramonium - blotchy local lesions developing slowly; not systemic.
• Cassia occidentalis - small local lesions; not systemic.

Diagnostically insusceptible host species

Beta vulgaris, Nicotiana glutinosa, N. tabacum, Petunia × hybrida, Zea mays.

Maintenance and propagation hosts

Datura stramonium, Cymbidium sp.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L), Cassia occidentalis (L), Datura stramonium (L).

Susceptible host species

• Cassia occidentalis
• Cattleya
• Chenopodium amaranticolor
• Cucumis sativus
• Cymbidium
• Datura stramonium
• Epidendrum
• Gomphrena globosa
• Laelia
• Laeliocattleya
• Oncidium
• Oryza sativa
• Phalaenopsis
• Tropaeolum majus
• Vanda
• Vanilla fragrans
• Zinnia elegans
• Zygopetalum

Insusceptible host species

• Apium graveolens
• Beta vulgaris
• Brassica campestris ssp. napus
• Brassica campestris ssp. pekinensis
• Brassica oleracea var. botrytis
• Capsicum frutescens
• Catharanthus roseus
• Cucurbita maxima
• Dianthus caryophyllus
• Glycine max
• Lactuca sativa
• Lathyrus odoratus
• Lycopersicon esculentum
• Nicotiana glutinosa
• Nicotiana tabacum
• Ocimum basilicum
• Petunia × hybrida
• Phaseolus vulgaris
• Raphanus sativus
• Trifolium incarnatum
• Vigna unguiculata
• Zea mays

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (1/2)
• Compositae (1/2)
• Cucurbitaceae (1/2)
• Gramineae (1/2)
• Leguminosae-Caesalpinioideae (1/1)
• Orchidaceae (10/10)
• Solanaceae (1/6)
• Tropaeolaceae (1 /1)

Families containing insusceptible hosts

• Apocynaceae (1/1)
• Caryophyllaceae (1/1)
• Chenopodiaceae (1/2)
• Compositae (1/2)
• Cruciferae (4/4)
• Cucurbitaceae (1/2)
• Gramineae (1/2)
• Labiatae (1/1)
• Leguminosae-Papilionoideae (5/5)
• Solanaceae (5 /6)
• Umbelliferae (1/1)

Comments on host-range

Some early reports of host range were probably of odontoglossum ringspot tobamovirus.

Sources of host-range data

Jensen (1955); Jensen and Gold (1955); Murakishi (1958); Kado and Jensen (1964); Faccioli and Marani (1979).

Physical and biochemical properties

Properties of particles in sap

TIP: 60-70 °C. LIV: 25 days. DEP: log₁₀ minus 6-7. Leaf sap contains many virions.

Purification method

Francki and McLean (1968).

Particle morphology

Virions filamentous; not enveloped; usually flexuous; with a clear modal length; of 480 nm; 13 nm wide. Axial canal obscure. Basic helix obvious; pitch of basic helix 2.8 nm.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 121 S.

Biochemical properties

Virions contain 5.6 % nucleic acid; 94 % protein; 0 % lipid.

Genome consists of RNA; single-stranded; linear. Total genome size 8.1 kb. Genome unipartite; largest (or only) genome part 8.1 kb. Genomic nucleic acid isolated by Frowd and Tremaine (1977). Base composition 21.1 % G; 28.9 % A; 24.4 % C; 25.6 % U.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• X62133 Em(40)_vi:CMVIRCP Gb(84)_vi:CMVIRCP Cymbidium mosaic virus gene for coat protein. 6/92 1,290bp.
• X62663 Em(40)_vi:CMV11KD Gb(84)_vi:CMV11KD Cymbidium mosaic virus RNA for 11Kd protein. 6/92 276bp.
• X62664 Em(40)_vi:CMV14KD Gb(84)_vi:CMV14KD Cymbidium mosaic virus RNA for 14Kd protein. 6/92 339bp
• X62665 Em(40)_vi:CMVCP Gb(84)_vi:CMVCP Cymbidium mosaic virus RNA for coat protein. 10/93 749bp. 4 sequences.
• X81051 Em(44)n:Cmpvcp Gb(90)_vi:Cmpvcp Cymbidium mosaic potexvirus genomic RNA for coat protein. 7/95 748bp.

Features of proteins

Virion protein(s) one; M_r 27600. Method of preparation: Francki and McLean (1968). Amino acid composition: Frowd and Tremaine (1977).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves; in cytoplasm. Inclusions cytoplasmic present in infected cells; are unusual in shape; banded bodies; they contain virions. Other cellular changes: osmiophilic globules in chloroplasts.

Taxonomy and relationships

Potexvirus

Virus(es) with serologically unrelated virions

White clover mosaic and potato X viruses.

Comments and References

References

• Faccioli, G. and Marani, F. (1979). Phytopathol. Medit. 18: 21.
• Francki, R.I.B. (1970). CMI/AAB Descr. Pl. Viruses No. 27, 3 pp.
• Francki, R.I.B. and McLean, G.D. (1968). Aust. J. biol. Sci. 21: 1311.
• Francki, R.I.B., Randles, J.W., Chambers, T.C. and Wilson, S.B. (1966). Virology 28: 729.
• Frowd, J.A. and Tremaine, J.H. (1977). Phytopathology 67: 43.
• Hanchey, P., Livingstone, C.H. and Reeves, F.B. (1975). Physiol. Pl. Path. 6: 227.
• Jensen, D.D. (1950). Phytopathology 40: 966.
• Jensen, D.D. (1955). Phytopathology 41: 401.
• Jensen, D.D. and Gold, A.H. (1955). Phytopathology 45: 327.
• Kado, C.I. and Jensen, D.D. (1964). Phytopathology 54: 974.
• Murakishi, H.H. (1958). Phytopathology 48: 132.
• Pearson, M.N., Brunt, A.A. and Pone, S.P. (1990). J. Phytopath. 128: 46.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.