Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Cucumber leaf spot carmovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by Sc. H. Kleinhempel and I. Weber, 1987.

Nomenclature

Synonyms

cucumber fruit streak virus (Gallitelli et al., 1983; Weber et al., 1986).

Acronym

CLSV

Strains

cucumber leaf spot (LS), cucumber fruit streak (FS).

ICTV decimal code

74.0.2.0.006

Host range and symptoms

Natural host range and symptoms

Symptoms disappear soon after infection.
• Cucumis sativus - chlorotic spots with necrotic centres.

Transmission

Transmitted by a vector; a fungus; Olpidium radicale; Chytridiales. Virus transmitted by mechanical inoculation; not transmitted by contact between plants; transmitted by seed.

Ecology and control

Studies reported by Weber et al. (1982); Gallitelli et al. (1983).

Geographical distribution

Spreads in Germany, Greece, Jordan, and the UK.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Celosia argentea, Gomphrena globosa, Chenopodium murale, C. quinoa, Nicotiana clevelandii, Petunia × hybrida - necrotic local lesions.

Diagnostically insusceptible host species

Phaseolus vulgaris var. `Pinto', Avena sativa, Datura stramonium, Lycopersicon esculentum, Nicotiana glutinosa.

Maintenance and propagation hosts

Cucumis sativus, Nicotiana megalosiphon.

Assay hosts (Local lesions or Whole plants)

Chenopodium quinoa (L).

Susceptible host species

• Celosia argentea
• Celosia cristata
• Chenopodium amaranticolor
• Chenopodium capitatum
• Chenopodium murale
• Chenopodium quinoa
• Cucumis melo
• Cucumis sativus
• Cucurbita maxima
• Cucurbita pepo
• Gomphrena globosa
• Luffa acutangula
• Nicotiana clevelandii
• Nicotiana megalosiphon
• Ocimum basilicum
• Petunia × hybrida
• Spinacia oleracea
• Vigna unguiculata
• Zinnia elegans

Insusceptible host species

• Apium graveolens
• Avena sativa
• Brassica oleracea var. botrytis
• Capsicum annuum
• Datura stramonium
• Lactuca sativa
• Lycopersicon esculentum
• Nicotiana glutinosa
• Nicotiana tabacum
• Phaseolus vulgaris
• Physalis floridana
• Pisum sativum
• Raphanus sativus
• Solanum melongena
• Triticum aestivum
• Vicia faba

Families containing susceptible hosts

• Amaranthaceae (3/3)
• Chenopodiaceae (5/5)
• Compositae (1/2)
• Cucurbitaceae (5/5)
• Labiatae (1/1)
• Leguminosae-Papilionoideae (1/4)
• Solanaceae (3/10)

Families containing insusceptible hosts

• Compositae (1/2)
• Cruciferae (2/2)
• Gramineae (2/2)
• Leguminosae-Papilionoideae (3/4)
• Solanaceae (7/10)
• Umbelliferae (1/1)

Sources of host-range data

Weber et al. (1982); Gallitelli et al. (1983).

Physical and biochemical properties

Properties of particles in sap

TIP: 80-85 °C. LIV: 20 days. DEP: log₁₀ minus 6-7. Leaf sap contains few virions (naturally), or contains many virions (artificially). Electron microscopy: ISEM is recommended.

Purification method

Weber et al. (1982).

Particle morphology

Virions isometric; not enveloped; c. 28 nm in diameter; rounded in profile; with a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 132 S (FS). Density 1.342 g cm^-3 in CsCl (LS), or 1.35 g cm^-3 in CsCl (FS).

Biochemical properties

Virions contain 20 % nucleic acid; 80 % protein.

Genome consists of RNA; single-stranded; linear. Total genome size 5.3 kb (LS), or 4.6 kb (FS). Genome unipartite; largest (or only) genome part 5.3 kb (LS), or 4.6 kb (FS). Genomic nucleic acid isolated by Leiser et al. (1981).

Features of proteins

Virion protein(s) one; M_r 44000 (LS), or 47000 (FS).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves, mesophyll and phloem; in cytoplasm. Inclusions present in infected cells; are crystals in the cytoplasm; they contain virions. Other cellular changes: mitochondria maybe deformed and the Golgi increased to form many small vesicles containing fine fibrils.

Taxonomy and relationships

Carmovirus: Tombusviridae

Virus(es) with serologically unrelated virions

Carnation mottle, cocksfoot mild mosaic, cucumber necrosis, melon necrotic spot, narcissus tip necrosis, tomato bushy stunt, tobacco necrosis (Weber et al., 1982), artichoke mottled crinkle, cymbidium ringspot, carnation mottle, cucumber necrosis, pelargonium leaf curl, southern bean mosaic, sowbane mosaic, tobacco necrosis and tomato bushy stunt viruses (Gallitelli et al., 1983).

Best tests for diagnosis

ELISA.

Comments and References

References

• Campbell, R.N., Lecoq, H., Wipf-Scheibel, C. and Sim, S.T. (1991). J. gen. Virol. 72: 3115.
• Gallitelli, D., Vovlas, C. and Avgelis, A. (1983). Phytopath. Z. 106: 149.
• Leiser, R.M., Stanarius, A., and Stanarius, Th. (1981). Arch. Phytopath. PflSchutz. 17: 299.
• Weber, I. (1986). CMI/AAB Descr. Pl. Viruses No. 319, 4 pp.
• Weber, I., Proll, E., Ostermann, W.D., Leiser, R.M., Stanarius, A. and Kegler, H. (1982). Arch. Phytopath. PflSchutz. 18: 137.
• Weber, I., Stenarius, A. and Kalinina, I. (1986). Arch. Phytopath. PflSchutz. 22: 169.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.