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Descriptions and Lists from the VIDE Database

Cucumber green mottle mosaic tobamovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by R.I.B. Francki, 1988.

Nomenclature

Synonyms

cucumber green mottle mosaic virus, tobacco mosaic virus watermelon strain - W, cucumber virus 3 (Ainsworth, 1935), cucumber virus 4 (Ainsworth, 1935; Hollings et al., 1975), bottlegourd Indian mosaic virus, cucumis virus 2.

Acronym

CGMMV

ICTV decimal code

71.0.1.0.002

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Cucumis sativus, Citrullus vulgaris, Lagenaria siceraria (bottlegourd) - mosaic.

Transmission

Transmitted by means not involving a vector, or a vector; an insect; Raphidopalpa fevicolis (Rao and Varma, 1984); Coleoptera. Virus transmitted by mechanical inoculation.

Geographical distribution

Spreads in the Eurasian region; India, Japan, and the UK.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Cucumis sativus, Citrullus vulgaris - systemic mosaic.

Diagnostically insusceptible host species

Datura stramonium, Petunia × hybrida.

Maintenance and propagation hosts

Cucumis sativus.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L), Cucumis sativus (W).

Susceptible host species

• Chenopodium amaranticolor
• Chenopodium murale
• Citrullus lanatus
• Citrullus vulgaris
• Cucumis sativus
• Lagenaria siceraria
• Nicotiana tabacum

Insusceptible host species

• Capsicum annuum
• Datura stramonium
• Nicotiana glutinosa
• Petunia × hybrida

Families containing susceptible hosts

• Chenopodiaceae (2/2)
• Cucurbitaceae (4/4)
• Solanaceae (1/5)

Families containing insusceptible hosts

• Solanaceae (4/5)

Physical and biochemical properties

Properties of particles in sap

TIP: 80-90 °C. LIV: more than 240 days (at 20ºC). DEP: log₁₀ minus 6. Leaf sap contains many virions.

Purification method

Tung and Knight (1972); Francki and McLean (1968).

Particle morphology

Virions rod-shaped; not enveloped; usually straight; with a clear modal length; of c 300 nm; c. 15 nm wide. Axial canal obvious. Basic helix obvious.

Biochemical properties

Virions contain c. 5 % nucleic acid; c. 95 % protein.

Genome consists of RNA; single-stranded; linear. Total genome size c. 6.5 kb. Genome unipartite; largest (or only) genome part 6.5 kb. Genomic nucleic acid isolated by Peden and Symons (1973). Base composition 23.2 % G (CGMMV-W, 25.8% (CV4), 25.5% (CV3)); 24.6 % A (CGMMV-W, 25.8% (CV4), 25.8% (CV3)); 20.6 % C (CGMMV-W, 19.3% (CV4), 18.3% (CV3)); 31.6 % U (CGMMV-W, 29.5% (CV4), 30.8% (CV3)). Infectivity decreased when deproteinised with proteases; retained when deproteinised with phenol or detergent. Poly A region absent. Additional factor not required for infectivity. Genome has tRNA-like activity. Genome accepts histidine. Nucleotide sequence references: Meshi et al. (1983).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• D12505 Em(40)_vi:MCGSHCG Gb(84)_vi:MCGSHCG Cucumber green mottle mosaic virus (CGMMV) complete genome. 10/92 6,421bp.
• J02054 Gb(84)_vi:MCGCPW cucumber green mottle mosaic virus coat protein gene. 9/83 1,071bp.
• J04322 Em(40)_vi:TOBMCG30 Gb(84)_vi:MCG30KP Cucumber green mottle mosaic virus 30K protein gene, complete cds, and 180K and coat protein 3 sequences.

Features of the genome

Features of the genome: the genomic RNA has been sequenced (Ugaki et al., 1991).

Non-genomic nucleic acid found in the virions; is subgenomic mRNA. Sub-genomic mRNA found in infected cells.

Features of proteins

Virion protein(s) one; M_r 17261; coat protein. Method of preparation: Fraenkel-Conrat (1957); Tung and Knight (1972). Amino acid sequence: Meshi et al. (1983). Amino acid composition: Nozu et al. (1971); Tung and Knight (1972).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves, mesophyll, epidermis, vascular parenchyma, xylem, phloem, companion cells and all parts of the host plant; in cytoplasm and in cell vacuoles. Inclusions present in infected cells; are crystals in the cytoplasm; they contain virions. Other cellular changes: vesiculation of mitochondria.

Taxonomy and relationships

Tobamovirus

Virus(es) with serologically related virions

Frangipani mosaic, kyuri green mottle mosaic, ribgrass mosaic, tobacco mosaic, tomato mosaic and tobacco mild green mosaic viruses.

Virus(es) with serologically unrelated virions

Odontoglossum ringspot, sunn-hemp mosaic viruses.

Additional comments on relationships

The host ranges of cucumber green mottle mosaic virus and kyuri green mottle mosaic virus are similar, and their coat proteins have greater amino acid sequence similarity than either has to those of other tobamoviruses, which are serologically more distantly related.

Comments and References

References

• Ainsworth, G.C. (1935). Ann. appl. Biol. 22: 55.
• Fraenkel-Conrat, H. (1957). Virology 4: 1.
• Francki, R.I.B. and McLean, G.D. (1968). Aust. J. biol. Sci. 21: 1311.
• Francki, R.I.B., Hu, J. and Palukaitis, P. (1986). Intervirology 26: 156.
• Hollings, M., Komuro, Y. and Tochihara, H. (1975). CMI/AAB Descr. Pl. Viruses No. 154, 4 pp.
• Meshi, T., Kiyama, R. Ohmi, T. and Okada, Y. (1983). Virology 127: 54.
• Nozu, Y., Tochihara, H., Komuro, Y. and Okada, Y. (1971). Virology 45: 577.
• Peden, K.W.C. and Symons, R.H. (1973). Virology 53: 487.
• Rao, D.C. and Varma, A. (1984). Phytopath. Z. 109: 325.
• Tung, J.S. and Knight, C.A. (1972). Virology 48: 574.
• Ugaki, M., Toriyama, M., Kakutani, T., Hidaka, S., Kiguchi, T., Nagata, R., Sato, T., Motoyoshi, F. and Nishiguchi, M. (1991). J. gen. Virol. 72: 1487.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.