Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Crimson clover latent nepovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by A.J. Cockbain, 1982. Revised 1983.

Nomenclature

Synonyms

clover Crimson latent virus.

Acronym

CCLV

ICTV decimal code

18.0.3.0.012

Host range and symptoms

Natural host range and symptoms

• Trifolium incarnatum - symptomless.

Transmission

Not transmitted by Myzus persicae. Virus transmitted by mechanical inoculation; not transmitted by contact between plants; transmitted by seed (97% in T. incarnatum, 20% in C. quinoa).

Geographical distribution

Found, but with no evidence of spread, in seed from France, Italy, The Netherlands, U.K. and the U.S.A.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor - systemic mosaic, leaf malformation.
• C. quinoa - chlorotic local lesions, systemic mosaic, ringspots, leaf malformation.

Diagnostically insusceptible host species

Cucumis sativus, Phaseolus vulgaris, Pisum sativum, Vicia faba, Nicotiana clevelandii, N. glutinosa, N. tabacum, Petunia × hybrida.

Maintenance and propagation hosts

Chenopodium album, C. amaranticolor, C. quinoa, Trifolium incarnatum.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor, C. quinoa.

Susceptible host species

• Chenopodium album
• Chenopodium amaranticolor
• Chenopodium quinoa
• Trifolium incarnatum

Insusceptible host species

• Beta vulgaris
• Brassica campestris ssp. pekinensis
• Chenopodium foetidum
• Chenopodium murale
• Cucumis sativus
• Glycine max
• Gomphrena globosa
• Lycopersicon esculentum
• Medicago sativa
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana tabacum
• Petunia × hybrida
• Phaseolus vulgaris
• Physalis floridana
• Pisum sativum
• Tetragonia tetragonioides
• Trifolium pratense
• Trifolium repens
• Trifolium subterraneum
• Vicia faba
• Vigna unguiculata
• Vigna unguiculata ssp. sesquipedalis

Families containing susceptible hosts

• Chenopodiaceae (3/6)
• Leguminosae-Papilionoideae (1/11)

Families containing insusceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (3/6)
• Cruciferae (1/1)
• Cucurbitaceae (1/1)
• Leguminosae-Papilionoideae (10/11)
• Solanaceae (6/6)
• Tetragoniaceae (1/1)

Sources of host-range data

Kenten et al. (1980).

Physical and biochemical properties

Properties of particles in sap

TIP: 60-65 °C. LIV: 20-30 days. DEP: log₁₀ minus 2-3. Leaf sap contains few virions.

Purification method

Kenten et al. (1980).

Particle morphology

Virions isometric; not enveloped; 26 nm in diameter; angular in profile; without a conspicuous capsomere arrangement.

Physical properties

Three sedimenting components in purified preparations; sedimentation coefficient of the fastest 122 S (B); of the other(s) 101 S (M), or 52 S (T). Density 1.5 g cm^-3 in CsCl (B), or 1.44 g cm^-3 in CsCl (M).

Biochemical properties

Virions contain 41 % nucleic acid (B), or 34 % nucleic acid (M), or 0 % nucleic acid (T); 59 % protein (B), or 66 % protein (M), or 100 % protein (T).

Genome consists of RNA; single-stranded. Total genome size 12.2 kb. Genome of two parts; largest (or only) genome part the larger 7.1 kb; the 2nd largest 5.2 kb. Additional factor not required for infectivity.

Features of proteins

Virion protein(s) one; M_r 52000.

Taxonomy and relationships

Nepovirus: Comoviridae

Virus(es) with serologically unrelated virions

Arabis mosaic, arracacha A, artichoke Italian latent, lucerne Australian latent, bean leaf roll, bean pod mottle, beet cryptic, broad bean stain, broad bean mottle, broad bean true mosaic, broad bean wilt, cherry leaf roll, cherry rasp leaf, cacao necrosis, grapevine Bulgarian latent, grapevine chrome mosaic, grapevine fanleaf, hibiscus latent ringspot, mulberry ringspot, myrobalan latent ringspot, pea enation mosaic, peach rosette mosaic, potato black ringspot, raspberry ringspot, red clover mottle, red clover necrotic mosaic, southern bean mosaic, strawberry latent ringspot, tobacco ringspot, tomato black ring and tomato ringspot viruses.

Comments and References

References

• Kenten, R.H., Cockbain, A.J. and Woods, R.D. (1980). Ann. appl. Biol. 96: 79.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.