Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Cowpea mottle (?) carmovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by R. Bozarth, 1980. Revised 1990.

Nomenclature

Acronym

CPMoV

ICTV decimal code

74.0.2.T.015

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Vigna unguiculata - mottling or mosaic.
• Vigna subterranea - green mottle, shortening of petioles.

Transmission

Transmitted by a vector; an insect; Ootheca mutabilis, Paraluperodes lineata; Coleoptera. Virus transmitted by mechanical inoculation; transmitted by seed (10% in V. unguiculata).

Geographical distribution

Spreads in Nigeria.

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show chlorotic local lesions, vein necrosis, mottling, malformation.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor, C. quinoa - diffuse chlorotic local lesions; not systemic.
• Cajanus cajan, Vigna unguiculata - severe systemic chlorotic or green mottle, leaf malformation. Necrosis in youngest primary leaves.
• Glycine max, Macroptilium lathyroides, Phaseolus acutifolius, P. lunatus, P. vulgaris, Vigna radiata, V. mungo - systemic mosaic.

Maintenance and propagation hosts

Vigna unguiculata.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L), C. quinoa (L), Vigna unguiculata (L).

Susceptible host species

• Cajanus cajan
• Chenopodium amaranticolor
• Chenopodium quinoa
• Glycine max
• Macroptilium lathyroides
• Macrotyloma uniflorum
• Nicotiana tabacum
• Phaseolus acutifolius
• Phaseolus lunatus
• Phaseolus vulgaris
• Vigna mungo
• Vigna radiata
• Vigna subterranea
• Vigna unguiculata

Insusceptible host species

• Atriplex hortensis
• Chenopodium murale
• Cucumis sativus
• Datura stramonium
• Lycopersicon esculentum
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana tabacum
• Petunia × hybrida
• Tetragonia tetragonioides
• Vicia faba
• Zinnia elegans

Families containing susceptible hosts

• Chenopodiaceae (2/4)
• Leguminosae-Papilionoideae (11/12)
• Solanaceae (1/6)

Families containing insusceptible hosts

• Chenopodiaceae (2/4)
• Compositae (1/1)
• Cucurbitaceae (1/1)
• Leguminosae-Papilionoideae (1/12)
• Solanaceae (6/6)
• Tetragoniaceae (1/1)

Sources of host-range data

Shoyinka et al. (1978).

Physical and biochemical properties

Properties of particles in sap

TIP: 60 °C. LIV: 1 days. DEP: log₁₀ minus 6 (x 2). Leaf sap contains many virions.

Purification method

Shoyinka et al. (1978).

Particle morphology

Virions isometric; not enveloped; 30 nm in diameter; rounded in profile; with a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 122 S. Density 1.3492 g cm^-3 in CsCl.

Biochemical properties

Virions contain 20 % nucleic acid; 80 % protein; 0 % lipid.

Genome consists of RNA; single-stranded. Total genome size 3.94 kb. Genome unipartite; largest (or only) genome part 3.94 kb.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• M65155 Em(40)_vi:MCCCP Gb(84)_vi:MCCCP Cowpea mottle virus capsid protein gene. 8/91 1,104bp. 1 sequence.
• U20976 Em(44)n:Cm20976 Gb(90)_vi:Cmu20976 Cowpea mottle virus polyprotein genes, complete cds.

Features of the genome

3 virus specified dsRNA species found in infected cells. Size of largest virus specified dsRNA 4.4 kbp; 2nd largest 1.9 kbp; 3rd largest 1.35 kbp.

Features of proteins

Virion protein(s) one; M_r 44500 (»1500); coat protein. Method of preparation: Bozarth and Shoyinka (1979).

Virus-coded non-virion proteins isolated; three proteins found. M_r of the largest 50000. M_r of 2nd largest 27000. M_r of 3rd 11000.

Replication

Replication does not depend on a helper virus.

Taxonomy and relationships

Possible Carmovirus: Tombusviridae

Virus(es) with serologically unrelated virions

Almond mosaic, apple mosaic, arabis mosaic, artichoke crinkle, bean mild mosaic, bean pod mottle, bean rugose mosaic, broad bean mottle virus, brome mosaic, carnation ringspot, cowpea severe mosaic, cucumber necrosis, cynosurus mottle, eggplant mosaic, elm mosaic, hibiscus chlorotic ringspot, okra mosaic, pelargonium ringspot, southern bean mosaic, sowbane mosaic, squash mosaic virus, tobacco ringspot, tobacco streak and tomato ringspot viruses.

Comments and References

References

• Bozarth, R.F. and Shoyinka, S.A. (1979). CMI/AAB Descr. Pl. Viruses No. 212, 3 pp.
• Kim, J.W. (1989). Ph.D. Thesis. Indiana State University, Terra Haute, Indiana, U.S.A.
• Shoyinka, S.A., Bozarth, R.F., Reese, J. and Rossel, H.W. (1978). Phytopathology 68: 693.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.