Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Cotton leaf crumple bigeminivirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by J.K. Brown, 1987 and A.A. Brunt, 1986.

Nomenclature

Acronym

CLCrV

Strains

Mild and severe strains recognised (Erwin and Meyer, 1961).

ICTV decimal code

29.0.3.0.012

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Species of Gossypium, Abutilon, Althaea, Hibiscus, Malva and of Castanospermum, Glycine, Phaseolus, Vicia - mosaic and malformation.

Transmission

Transmitted by a vector; an insect; Bemisia tabaci; Aleyrodidae. Transmitted in a semi-persistent manner. Virus lost by the vector when it moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector; not transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Ecology and control

Studies reported by Brown et al. (1983); Brown et al. (1987); Duffus and Flock (1982); Erwin and Meyer (1961); Brown et al. (1986).

Geographical distribution

Spreads in the Middle East; India, Mexico, and the USA.

Experimental host range

Few (<3) families susceptible. Experimentally infected plants mostly show chlorosis and malformation.

Diagnostically susceptible host species and symptoms

• Malva parviflora, Gossypium hirsutum `Delta Pine 70', Vigna angularis (Azuki bean) and Castanospermum australe (Delgado bean) - systemic chlorosis and malformation.

Diagnostically insusceptible host species

Cucumis melo, Abelmoschus esculentus, Spinacia oleracea, Nicotiana glutinosa, Zinnia elegans.

Maintenance and propagation hosts

Gossypium hirsutum, Malva parviflora and a useful purification host is Phaseolus vulgaris.

Assay hosts (Local lesions or Whole plants)

Gossypium hirsutum (W), Malva parviflora (W).

Susceptible host species

• Abutilon
• Althaea
• Castanospermum
• Castanospermum australe
• Glycine
• Glycine max
• Gossypium
• Gossypium hirsutum
• Hibiscus
• Hibiscus cannabinus
• Malva
• Malva parviflora
• Phaseolus
• Phaseolus acutifolius
• Phaseolus vulgaris
• Vicia
• Vigna angularis

Insusceptible host species

• Abelmoschus esculentus
• Cucumis melo
• Nicotiana glutinosa
• Spinacia oleracea
• Zinnia elegans

Families containing susceptible hosts

• Leguminosae-Papilionoideae (9/9)
• Malvaceae (8/9)

Families containing insusceptible hosts

• Chenopodiaceae (1/1)
• Compositae (1/1)
• Cucurbitaceae (1/1)
• Malvaceae (1/9)
• Solanaceae (1/1)

Sources of host-range data

Brown and Nelson (1984); Brown et al. (1986).

Physical and biochemical properties

Properties of particles in sap

Leaf sap contains few virions. Electron microscopy: fixation with glutaraldehyde is essential. UA is better than PTA.

Purification method

Brown and Nelson (1984).

Particle morphology

Virions geminate; not enveloped; 17-20 nm in diameter; dimers 30-32 nm in length.

Cytopathology

Virions found in phloem. Inclusions present in infected cells (in phloem and parenchyma only); are unusual in shape; spherical or amorphous. They are more numerous in younger leaves. Other cellular changes: hypertrophy of cells containing inclusions.

Taxonomy and relationships

bigeminivirus: Geminiviridae

Comments and References

References

• Brown, J.K. and Nelson, M.R. (1984). Phytopathology 74: 987.
• Brown, J.K. and Nelson, M.R. (1987). Plant Dis. 71: 522.
• Brown, J.K., Mihail, J.D. and Nelson, M.R. (1987). Plant Dis. 71: 699.
• Brown, J.K., Butler, G.D. and Nelson, M.R. (1983). Phytopathology 73: 787.
• Brown, J.K., Nelson, M.R. and Lambe, R.C. (1986). Plant Dis. 70: 981.
• Dickson, R.C., Johnson, M.M. and Laird, E. (1954). Phytopathology 44: 479.
• Duffus, J.E. and Flock, R.A. (1982). Calif. Agric. 36: 4.
• Erwin, D.C. and Meyer, R. (1961). Phytopathology 51: 472.
• Tsao, P.M. (1963). Phytopathology 53: 243.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.