Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Citrus leaf rugose ilarvirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by P. Barkley, 1987. Checked by R.F. Lee, 1989.

Nomenclature

Synonyms

citrus psorosis virus complex, citrus crinkly leaf ilarvirus.

Acronym

CiLRV

ICTV decimal code

10.0.2.02.03

Host range and symptoms

Natural host range and symptoms

Symptoms persist, or vary seasonally.
• Citrus aurantifolia, C. limon, C. paradisi - flecking of young leaves and malformation of old leaves (particularly of Eureka lemons), fruit small and lumpy.
• Phaseolus vulgaris cv. Red Kidney - necrotic local lesions, no systemic infection.

Transmission

Transmitted by means not involving a vector. Virus transmitted by mechanical inoculation; transmitted by grafting; possibly transmitted by seed.

Geographical distribution

Spreads in the USA (California). Found, but with no evidence of spread, in Australia.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Citrus limon Eureka lemon - asteroid local lesions.
• Citrus sinensis, C. limon, C. reticulata - crinkling and flecking.
• Vigna unguiculata - vein clearing, malformation.
• Crotalaria spectabilis - systemic chlorotic veinbanding.

Maintenance and propagation hosts

any Citrus species.

Assay hosts (Local lesions or Whole plants)

Vigna unguiculata cv. `Blackeye' No. 5. (L).

Susceptible host species

• Catharanthus roseus
• Citrus aurantifolia
• Citrus limon
• Citrus paradisi
• Citrus reticulata
• Citrus sinensis
• Crotalaria spectabilis
• Phaseolus vulgaris (cv. Red Kidney)
• Vigna unguiculata

Families containing susceptible hosts

• Apocynaceae (1/1)
• Leguminosae-Papilionoideae (3/3)
• Rutaceae (5/5)

Purification method

Yot-Dauthy and Bové (1968); Garnsey (1975).

Physical and biochemical properties

Particle morphology

Virions unusually shaped; irregularly isometric virions; 25-32 nm in diameter (Yot-Dauthy and Bové, 1968).

Physical properties

Four sedimenting components in purified preparations; sedimentation coefficient 105 S; of the other(s) 98 S (also 89 and 79). A₂₆₀/A₂₈₀ ratio 1.4-1.46 (mixed components).

Biochemical properties

Genome consists of RNA; single-stranded; linear. Total genome size 7.888 kb. Genome of three parts; largest (or only) genome part 3.099 kb; the 2nd largest 2.817 kb; the 3rd largest 1.972 kb.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• U17390 Em(43)_vi:Cl17390 Gb(89)_vi:Clu17390 Citrus leaf rugose virus RNA 3 movement protein and coat protein genes, complete cds. 4/95 2,
• U17726 Em(43)_vi:Cl17726 Gb(89)_vi:Clu17726 Citrus leaf rugose virus RNA-2, polymerase gene, complete cds. 2/95 2,990bp.
• U23715 Em(44)n:Cl23715 Gb(90)n:Clu23715 Citrus leaf rugose ilarvirus putative viral replicase gene, complete cds. 8/95 3,404bp.

Features of the genome

Non-genomic nucleic acid found in the virions; is subgenomic mRNA; of the coat protein.

Features of proteins

Virion protein(s) one; M_r 26000; coat protein.

Taxonomy and relationships

Ilarvirus: Bromoviridae (subgroup 2)

Comments and References

References

• Attathom, S., Weathers, L.G. and Greer, F.C. (1975). Proc. Am. Phytopath. Soc. 2: 111.
• Fawcett, H.S. (1936). In: Citrus Diseases and their Control. McGraw Hill, New York, London, 656 pp.
• Garnsey, S.M. (1975). Phytopathology 65: 50.
• Gonsalves, D. and Garnsey, S.M. (1974). Virology 61: 343.
• Gonsalves, D. and Garnsey, S.M. (1975a). Virology 64: 23.
• Gonsalves, D. and Garnsey, S.M. (1975b). Virology 67: 311.
• Gonsalves, D. and Garnsey, S.M. (1975c). Virology 67: 319.
• Gonsalves, D. and Garnsey, S.M. (1976). CMI/AAB Descr. Pl. Viruses No. 164, 4pp.
• Yot-Dauthy, D. and Bové, J.M. (1968). Proc. 4th Conf. Int. Org. Citrus Virol., p. 255.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.