Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Cauliflower mosaic caulimovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by R.G. Garrett, 1982.

Nomenclature

Synonyms

brassica virus 3, broccoli mosaic virus, cabbage mosaic virus, cabbage virus B.

Acronym

CaMV

ICTV decimal code

15.0.1.0.004

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Arabidopsis thaliana, Brassica spp., Raphanus spp. and other species of the Brassicaceae, Resedaceae - vein-clearing or banding mosaic.

Transmission

Transmitted by a vector; an insect; Brevicoryne brassicae, Myzus persicae and at least 25 other species; Aphididae. Transmitted in a semi-persistent manner. Virus lost by the vector when it moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector; transmitted by mechanical inoculation.

Geographical distribution

Probably distributed worldwide.

Experimental host range

Few (<3) families susceptible. Experimentally infected plants mostly show vein clearing, mosaic.

Diagnostically susceptible host species and symptoms

• Brassica oleracea var. botrytis, B. campestris - vein clearing, mosaic.
• Brassica campestris ssp. napus - mosaic.

Maintenance and propagation hosts

Brassica campestris cv. Just Right.

Assay hosts (Local lesions or Whole plants)

Brassica campestris cv. Just Right.

Susceptible host species

• Arabidopsis thaliana
• Brassica campestris
• Brassica campestris ssp. chinensis
• Brassica campestris ssp. napus
• Brassica campestris ssp. pekinensis
• Brassica campestris ssp. rapa
• Brassica oleracea
• Brassica oleracea var. botrytis
• Brassicaceae
• Datura stramonium
• Nicotiana clevelandii
• Raphanus
• Resedaceae

Insusceptible host species

• Nicotiana bigelovii
• Nicotiana debneyi
• Nicotiana glutinosa

Families containing susceptible hosts

• Cruciferae (9/9)
• Resedaceae
• Solanaceae (2/5)

Families containing insusceptible hosts

• Solanaceae (3/5)

Sources of host-range data

Tompkins (1937); Broadbent and Tinsley (1953).

Physical and biochemical properties

Properties of particles in sap

TIP: 75-80 °C. LIV: 7-15 days. DEP: log₁₀ minus 3. Infectivity of sap not changed by treatment with di-ethyl ether. Leaf sap contains few virions. Electron microscopy: no special treatment required but virions flatten.

Purification method

Gardner and Shepherd (1980).

Particle morphology

Virions isometric; not enveloped; 50 nm in diameter; rounded in profile; without a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 208 S (Hull et al., 1976). Density 1.37 g cm^-3 in CsCl.

Biochemical properties

Virions contain 16-17 % nucleic acid; 83-84 % protein; 0 % lipid.

Genome consists of DNA; double-stranded; circular (linear molecules found but not infective). Total genome size 8.032 kb (pairs). Genome unipartite; largest (or only) genome part 8.032 kb (pairs). Genomic nucleic acid isolated by Shepherd et al. (1970). Base composition of ds genome 43 % (G + C). Poly A region absent. Nucleotide sequence references: Franck et al. (1980); Gardner et al. (1981).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• A00001 Em(40)_vi:A00001 Gb(84)_pat:A00001 Cauliflower mosaic virus satellite cDNA. 1/93 335bp.
• D00335 Em(40)_vi:MCAB1G6 Gb(84)_vi:MCAB1G6 Cauliflower mosaic virus (strain Bari 1), gene VI and flanks. 3/94 1,908bp.
• L09053 Em(40)_vi:MCAMIBP. Gb(84)_vi:MCAMIBP. Cauliflower mosaic virus major inclusion body protein (VI) gene, complete cds. 11/93 3,036bp.
• M10166 Em(40)_vi:MCAIBP Gb(84)_vi:MCA4184P Cauliflower mosaic virus IBP. gene promoter region. 7/89 92bp
• M10326 Em(40)_vi:MCA41841 Gb(84)_vi:MCA41841 Cauliflower mosaic virus (strain CM4-184) 5´ region, segment 1. 7/89 69bp.
• M10327 Em(40)_vi:MCA41842 Gb(84)_vi:MCA41842 Cauliflower mosaic virus (strain CM4-184) 5´ region, segment 2. 7/89 135bp.
• M10376 Em(40)_vi:MCACGDH Gb(84)_vi:MCACGDH Cauliflower mosaic virus (altered virulence isolate D/H), complete genome. 5/94 8,016bp.
• M10385 Em(40)_vi:MCA4184D Gb(84)_vi:MCA4184D Cauliflower mosaic virus (strain CM4-184) deletion mutant DNA. 7/89 90bp.
• M13776 Em(40)_vi:MCAIS Gb(84)_vi:MCAIS Cauliflower mosaic virus (CaMV) in vitro initiation sites. 7/89 320bp.
• M14412 Em(40)_sy:AG5MCA Gb(84)_sy:SYNTRN5MCA Synthetic gene of Cauliflower mosaic virus promoter and Transposon Tn5 kanamycin resistant ge
• M14790 Gb(84)_vi:MCAREPREC Cauliflower mosaic virus (strain CM4-184) U5 region DNA. 5/87 2,539bp.
• M16239 Em(40)_vi:MCA4184P Gb(84)_sy:SYN4184P1 Cauliflower mosaic virus IBP. gene promoter region with oligomer BamHI linker. 7/89 67bp
• M17415 Em(40)_vi:MCAATFA Gb(84)_vi:MCAATFA Cauliflower mosaic virus (CAMV) BstEII/XhoI aphid transmissibility fragment DNA. 7/89 1,556bp.
• M19736 Em(40)_sy:AG35S Gb(84)_sy:SYNMCA35S Cauliflower mosaic virus (CaMV) 35S promoter gene under Tn10-encoded tet repressor-operator c
• M19740 Em(40)_vi:MCACPPG2 Gb(84)_vi:MCACPPG2 Cauliflower mosaic virus viable recombinant DNA, 3´ flank to ORF (VIII,IV,V,V*) region. 7/89
• M19741 Em(40)_vi:MCACPPG1 Gb(84)_vi:MCACPPG1 Cauliflower mosaic virus viable recombinant ORF VIII mRNA, 3´ end; ORF V* mRNA, complete cds;
• M23620 Em(40)_vi:MCAMV6 Gb(84)_vi:MCAMV6 Cauliflower mosaic virus (CaMV) gene six protein gene, complete cds. 12/93 1,904bp.
• M32808 Em(40)_vi:MCAORFIX Gb(84)_vi:MCAORFIX1 Cauliflower mosaic virus unidentified protein gene, partial cds. 6/92 74bp.
• M32809 Em(40)_vi:MCAAAF1 Gb(84)_vi:MCAAAF1 Cauliflower mosaic virus aphid acquisition factor 5´ end. 6/92 229bp.
• M32810 Em(40)_vi:MCAORFX2 Gb(84)_vi:MCAORFX2 Cauliflower mosaic virus unidentified protein gene, 5´ end. 6/92 178bp.
• M32811 Em(40)_vi:MCAMPX Gb(84)_vi:MCAMPX Cauliflower mosaic virus matrix protein (put.), partial cds. 6/92 972bp.
• M32812 Em(40)_vi:MCACTCTP Gb(84)_vi:MCACTCTP Cauliflower mosaic virus cell-to-cell transport protein, 5´ end. 6/92 221bp.
• M32813 Em(40)_vi:MCAAAFCP Gb(84)_vi:MCAAAFCP Cauliflower mosaic virus aphid acquisition factor (3´ end), and coat protein (5´ end) genes.
• M32814 Em(40)_vi:MCACPX Gb(84)_vi:MCACPX Cauliflower mosaic virus coat protein, partial cds. 6/92 106bp
• M32815 Em(40)_vi:MCARTRA Gb(84)_vi:MCARTRA Cauliflower mosaic virus reverse transcriptase (V) gene, partial cds. 6/92 467bp.
• M37581 Em(40)_vi:MCAORF01 Gb(84)_vi:MCAORFIIPV Cauliflower mosaic virus ORF II protein gene, complete cds. 9/90 649bp.
• M37582 Em(40)_vi:MCAORFII Gb(84)_vi:MCAORFIICM Cauliflower mosaic virus ORF II protein gene, complete cds. 9/90 648bp.
• M74305 Em(40)_sy:AGCAMVCM5 Gb(84)_sy:SYNCAMVCM5 Synthetic expression vector pUC18cpPRV, constructed from cauliflower mosaic virus 35S gene-re
• M74306 Em(40)_sy:AGCAMVCM3 Gb(84)_sy:SYNCAMVCM3 Synthetic expression vector pUC18cpPRV constructed from cauliflower mosaic virus 35S gene-reg
• M90541 Em(40)_vi:MCAMATCAP Gb(84)_vi:MCAMATCAP Cauliflower mosaic virus movement protein, aphid transmission protein, DNA binding protein, c
• M90542 Em(40)_vi:MCAMADCRI Gb(84)_vi:MCAMADCRI Cauliflower mosaic virus movement protein gene, aphid transmission protein gene, DNA binding
• M90543 Em(40)_vi:MCACOMGEN Gb(84)_vi:MCACOMGEN Cauliflower mosaic virus movement protein gene, aphid transmission protein gene, DNA binding
• M94887 Em(40)_vi:MCAORFS Gb(84)_vi:MCAORFS Cauliflower mosaic virus DNA sequence, ORFs I, II, and VII. 10/92 1,692bp.
• S51055 Em(40)_vi:S51055 Gb(84)_vi:S51055 19S (promoter) cauliflower mosaic virus, Genomic, 240 nt. 11/93 240bp.
• S51061 Em(40)_vi:S51061 Gb(84)_vi:S51061 35S (promoter) cauliflower mosaic virus, Genomic, 210 nt. 11/93 210bp.
• V00140 Em(40)_vi:CAMVG1 Gb(84)_vi:CAMVG1 Cauliflower mosaic virus genome. 9/93 8,031bp.
• V00141 Em(40)_vi:CAMVG2 Gb(84)_vi:CAMVG2 Cauliflower mosaic virus genome. 9/93 8,024bp.
• X02606 Em(40)_vi:CACM4184 Gb(84)_vi:CACM4184 Cauliflower mosaic virus (CM4184) R, U5 and flanking regions. 7/86 2,539bp.
• X04879 Em(40)_sy:ARCAMVPR Gb(84)_sy:ARCAMVPR CaMV promoter in pMON273 vector (transgenic petunia) CaMV = Cauliflower Mosaic Virus 35S prom
• X14911 Em(40)_vi:CAMVI Gb(84)_vi:CAMVI Cauliflower mosaic virus gene VI for the inclusion body matrix protein. 9/93 3,131bp.
• X53860 Em(40)_vi:CMVVIG Gb(84)_vi:CMVVIG Cauliflower mosaic virus (CaMV) gene VI for viroplasmin. 9/93 2,283bp.
• X79465 Em(43)_vi:Cmvdna Gb(89)n:Cmvdna Cauliflower Mosaic Virus strain B29 complete DNA sequence. 5/95 8,031bp.

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

Virion protein(s) one; M_r 44000, or 58000. Method of preparation: Al Ani et al. (1979); Hahn and Shepherd (1980). Amino acid composition: Brunt et al. (1975); protein is basic because of relatively high lysine and arginine content.

Replication

Genome replicates in nuclei. Coat protein mRNA translated in the cytoplasm. Replication does not depend on a helper virus.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm; plasmodesmata. Inclusions present in infected cells; are unusual in shape; electron-dense matrices; they contain virions. Other cellular changes: deeply lobed nuclei, cell wall protrusions associated with vesicles and convoluted tubules.

Taxonomy and relationships

Caulimovirus (the type species)

Virus(es) with serologically related virions

Dahlia mosaic, strawberry vein-banding and carnation etched ring viruses.

Comments and References

References

• Al Ani, R., Pfeiffer, P. and Lebeurier, G. (1979). Virology 93: 188.
• Broadbent, L. and Tinsley, T.W. (1953). Pl. Path. 2: 88.
• Brunt, A.A., Barton, R.J., Tremaine, J.H. and Stace-Smith, R. (1975). J. gen. Virol. 27: 101.
• Cheng, R.H., Olron, N.H. and Barker, T.S. (1992). Virology 186: 655.
• Covey, S.N. and Hull, R. (1985). Oxford Surveys of Pl. Mol. Cell Biol. 2: 339.
• Donson, J. and Hull, R. (1983). J. gen. Virol. 64: 2281.
• Franck, A., Guilley, H., Jonard, G., Richards, K. and Hirth, L. (1980). Cell 21: 285.
• Gardner, R.C. and Shepherd, R.J. (1980). Virology 106: 159.
• Gardner, R.C., Howarth, A.J., Hahn, P., Brown-Luedi, M., Shepherd, R.J. and Messing, J. (1981). Nucl. Acids Res. 9: 2871.
• Hahn, P. and Shepherd, R.J. (1980). Virology 107: 295.
• Hull, R. and Covey, S.N. (1983). Trends Biochem. Sci. 8: 119.
• Kruse, J., Timmians, P. and Witz, J. (1987). Virology 159: 166.
• Meagher, R.B., Shepherd, R.J. and Boyer, H.W. (1977). Virology 80: 362.
• Pfeiffer, P. and Hohn, T. (1983). Cell 33: 781.
• Shepherd, R.J. (1970). CMI/AAB Descr. Pl. Viruses No. 24, 4 pp.
• Shepherd, R.J. (1981). CMI/AAB Descr. Pl. Viruses No. 243, 6 pp.
• Tompkins, C.M. (1937). J. Agric. Res. 55: 33.

Illustrations

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.