Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Cassia yellow blotch bromovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by J.L. Dale, 1984. Revised 1987.

Nomenclature

Acronym

CYBV

ICTV decimal code

10.0.3.0.004

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Cassia pleurocarpa - yellow leaf blotches.

Transmission

Virus transmitted by mechanical inoculation.

Geographical distribution

Spreads in Australia.

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show chlorotic local lesions, mottle, tip necrosis.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor, C. quinoa - chlorotic local lesions; not systemic.
• Cassia hirsuta, C. occidentalis, Trifolium incarnatum - systemic chlorotic mottle.
• Cicer arietinum - tip necrosis.
• Nicotiana clevelandii - systemic green mottle.

Diagnostically insusceptible host species

Arachis hypogaea, Pisum sativum, Vigna radiata.

Maintenance and propagation hosts

Nicotiana clevelandii.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L).

Susceptible host species

• Cassia hirsuta
• Cassia occidentalis
• Cassia pleurocarpa
• Cassia tora
• Chenopodium amaranticolor
• Chenopodium quinoa
• Cicer arietinum
• Cucumis sativus
• Desmodium tortuosum
• Glycine max
• Gomphrena globosa
• Lathyrus odoratus
• Lupinus angustifolius
• Macroptilium lathyroides
• Nicotiana clevelandii
• Phaseolus vulgaris
• Trifolium incarnatum
• Zea mays

Insusceptible host species

• Arachis hypogaea
• Avena sativa
• Cajanus cajan
• Cicer arietinum
• Clitoria ternatea
• Datura stramonium
• Helianthus annuus
• Hordeum vulgare
• Medicago sativa
• Pisum sativum
• Sorghum bicolor
• Trifolium pratense
• Triticum aestivum
• Vigna radiata
• Vigna unguiculata ssp. sesquipedalis

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (2/2)
• Cucurbitaceae (1/1)
• Gramineae (1/5)
• Leguminosae-Caesalpinioideae (4/4)
• Leguminosae-Papilionoideae (8/16)
• Solanaceae (1/2)

Families containing insusceptible hosts

• Compositae (1/1)
• Gramineae (4/5)
• Leguminosae-Papilionoideae (9/16)
• Solanaceae (1/2)

Sources of host-range data

Dale et al. (1984).

Physical and biochemical properties

Properties of particles in sap

Leaf sap contains many virions. Electron microscopy: best with UA or AM.

Purification method

Bancroft et al. (1967); Dale et al. (1984).

Particle morphology

Virions isometric; not enveloped; 26 nm in diameter; rounded in profile; without a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations (at pH 7); sedimentation coefficient 85 S. Isoelectric point pH 3.6.

Biochemical properties

Genome consists of RNA; single-stranded. Total genome size 8.7 kb. Genome of three parts; largest (or only) genome part the largest 3.4 kb; the 2nd largest 3 kb; the 3rd largest 2.3 kb. Genomic nucleic acid isolated by Dale et al. (1984). Base composition 24.4 % G; 24.9 % A; 22.2 % C; 28.5 % U. Infectivity retained when deproteinised with phenol or detergent. Additional factor not required for infectivity.

Features of the genome

Non-genomic nucleic acid found in the virions; is subgenomic mRNA.

Features of proteins

Virion protein(s) one; M_r 20800. Amino acid composition: Dale et al. (1984).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm and in nuclei. Inclusions absent from infected cells.

Taxonomy and relationships

Bromovirus: Bromoviridae

Virus(es) with serologically related virions

Broad bean mottle, brome mosaic and cowpea chlorotic mottle viruses.

Comments and References

References

• Bancroft, J.B., Hills, G.J. and Markham, R. (1967). Virology 31: 354.
• Dale, J.L. (1988). CMI/AAB Descr. Pl. Viruses No. 334, 3 pp.
• Dale, J.L., Gibbs, A.J. and Behncken, G.M. (1984). J. gen. Virol. 65: 281.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.