Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Carnation yellow stripe (?) necrovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by D. Gallitelli, 1991.

Nomenclature

Acronym

CYSV

ICTV decimal code

44.0.1.T.004

Host range and symptoms

Natural host range and symptoms

• Dianthus caryophyllus cv. Pink Shocking - yellow leaf stripes.

Transmission

Virus transmitted by mechanical inoculation.

Geographical distribution

Spreads in Italy.

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show chlorotic or necrotic local lesions.

Diagnostically susceptible host species and symptoms

• Chenopodium quinoa, C. amaranticolor, C. album - small chlorotic local lesions, becoming necrotic.
• Chenopodium ambrosioides - bright red local lesions.
• Gomphrena globosa - red-rimmed necrotic local lesions.
• Vigna unguiculata - systemic mosaic, chlorotic vein-banding.
• Saponaria vaccaria - chlorotic local lesions.

Diagnostically insusceptible host species

Hordeum vulgare, Cucumis sativus, Nicotiana benthamiana, N. rustica, Vigna radiata.

Maintenance and propagation hosts

Vigna unguiculata.

Assay hosts (Local lesions or Whole plants)

Vigna unguiculata (W), Chenopodium quinoa (L), C. amaranticolor (L), Gomphrena globosa (L), Dianthus barbatus (L).

Susceptible host species

• Chenopodium album
• Chenopodium amaranticolor
• Chenopodium ambrosioides
• Chenopodium quinoa
• Dianthus barbatus
• Dianthus caryophyllus
• Gomphrena globosa
• Lupinus albus
• Nicotiana clevelandii
• Saponaria vaccaria
• Vicia faba
• Vigna unguiculata

Insusceptible host species

• Avena sativa
• Cucumis sativus
• Cucurbita pepo
• Dianthus barbatus
• Hordeum vulgare
• Nicotiana benthamiana
• Nicotiana rustica
• Triticum durum
• Vigna radiata
• Zea mays

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Caryophyllaceae (3/3)
• Chenopodiaceae (4/4)
• Leguminosae-Papilionoideae (3/4)
• Solanaceae (1/3)

Families containing insusceptible hosts

• Caryophyllaceae (1/3)
• Cucurbitaceae (2/2)
• Gramineae (4/4)
• Leguminosae-Papilionoideae (1/4)
• Solanaceae (2/3)

Sources of host-range data

Gallitelli et al. (1979).

Physical and biochemical properties

Properties of particles in sap

TIP: 80 °C. LIV: 60 days. DEP: log₁₀ minus 7. Leaf sap contains many virions.

Purification method

Gallitelli et al. (1979).

Particle morphology

Virions isometric; 30 nm in diameter; angular in profile; without a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 99-105 S. Density 1.369 g cm^-3 in CsCl. A₂₆₀/A₂₈₀ ratio 1.61.

Biochemical properties

Virions contain 18 % nucleic acid; 72 % protein; 0 % lipid.

Genome consists of RNA; single-stranded. Total genome size 4 kb. Genome unipartite; largest (or only) genome part 4 kb. Genomic nucleic acid isolated by Gallitelli: phenol-SDS.

Features of proteins

Virion protein(s) one; M_r 27000; coat protein. Method of preparation: Laemmli (1970).

Cytopathology

Virions found in leaves and mesophyll; in cytoplasm. Inclusions present in infected cells; are crystals in the cytoplasm and membranous bodies; they do not contain virions.

Taxonomy and relationships

Possible Necrovirus

Virus(es) with serologically related virions

Tobacco necrosis virus (grape strain).

Best tests for diagnosis

Inoculation onto Chenopodium quinoa and serology.

Comments and References

References

• Gallitelli, D., Castellano, M.A., Di Franco, A. and Rana, G.L. (1979). Phytopathol. Medit. 18: 31.
• Laemmli, U.K. (1970). Nature, Lond. 227: 680.
• Rana, G.L., Di Franco, A. and Castellano, M.A. (1977). Ann. Fac. Agr. Univ. Bari, 29: 397.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.