Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Carnation latent carlavirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by J.R. Moran, 1987.

Nomenclature

Synonyms

dulcamara A virus, dulcamara B virus (Phillips and Brunt, 1981).

Acronym

CLV

ICTV decimal code

14.0.1.0.001

Host range and symptoms

Natural host range and symptoms

• Dianthus caryophyllus - few or no symptoms.

Transmission

Transmitted by a vector; an insect; Myzus persicae; Aphididae. Transmitted in a non-persistent manner. Virus transmitted by mechanical inoculation (but sometimes is difficult to transmit from carnation because its sap contains inhibitors of infection); not transmitted by contact between plants; not transmitted by seed.

Geographical distribution

Probably distributed worldwide (wherever carnations are grown).

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show local lesions, mottle.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor, C. quinoa - small chlorotic local lesions; systemic mottle.

Maintenance and propagation hosts

Dianthus barbatus.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L); C. quinoa (L).

Susceptible host species

• Beta vulgaris
• Chenopodium amaranticolor
• Chenopodium quinoa
• Dianthus barbatus
• Dianthus caryophyllus
• Gynura aurantiaca
• Nicotiana clevelandii

Families containing susceptible hosts

• Caryophyllaceae (2/2)
• Chenopodiaceae (3/3)
• Compositae (1/1)
• Solanaceae (1/1)

Physical and biochemical properties

Properties of particles in sap

TIP: 60-65 °C. LIV: 2-3 days. DEP: log₁₀ minus 3-4. Leaf sap contains few virions.

Purification method

Wetter and Paul (1961).

Particle morphology

Virions filamentous; not enveloped; usually straight (or slightly curved); with a clear modal length; of 650 nm; 12 nm wide. Axial canal obscure. Basic helix obscure; pitch of basic helix 3.3 nm.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 167 S.

Biochemical properties

Virions contain 6 % nucleic acid.

Genome consists of RNA; single-stranded. Total genome size 8.5 kb. Genome unipartite. Genomic nucleic acid isolated by Wetter and Milne (1981). Nucleotide sequence references: Haylor et al. (1990). The nucleotide sequence of the 3´ proximal region of the genomic RNA has been reported.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• X55897 Em(40)_vi:CLVTUCRF Gb(84)_vi:CLVTUCRF Carnation latent virus (CLV) 3´ terminal ORF. 9/93 393bp. 1 sequence.

Features of proteins

Virion protein(s) one; M_r 32000. Method of preparation: Paul (1973).

Taxonomy and relationships

Carlavirus (type member)

Virus(es) with serologically related virions

Cactus 2, chrysanthemum B, helenium S, hop latent, lilac mottle, lily symptomless, mulberry latent, muskmelon vein necrosis, nerine latent, passiflora latent, pea streak, potato M, potato S and red clover vein mosaic viruses.

Virus(es) with serologically unrelated virions

Narcissus latent, poplar mosaic, shallot latent and honeysuckle latent viruses.

Best tests for diagnosis

Use ISEM decoration to distinguish carnation latent from carnation vein mottle virus.

Comments and References

References

• Haylor, M.T., Brunt, A.A. and Coutts, R.H.A. (1990). Nucl. Acids Res. 18: 6127.
• Kassanis, B. (1954). Nature, Lond. 173: 109.
• Kassanis, B. (1955). Ann. appl. Biol. 43: 103.
• Kassanis, B. (1958). Ann. appl. Biol. 43: 103.
• Paul, H.L. (1974). Phytopath. Z. 80: 330.
• Phillips, S. and Brunt, A.A. (1981). Rep. Glasshouse Crops Res. Inst. 1980, p. 152.
• Wetter, C. (1971). CMI/AAB Descr. Pl. Viruses No. 61, 3 pp.
• Wetter, C. and Milne, R.G. (1981). In: Handbook of Plant Virus Infections and Comparative Diagnosis, p 695; ed. E. Kurstak. Elsevier, Amsterdam.
• Wetter, C. and Paul, H.L. (1961). Phytopath. Z. 43: 207.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.