Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Broccoli necrotic yellows cytorhabdovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by C. Büchen-Osmond, 1986.

Nomenclature

Acronym

BNYV

ICTV decimal code

62.0.4.0.003

Host range and symptoms

Natural host range and symptoms

• Brassica oleracea var. botrytis (broccoli), Brassica oleracea var. gemmifera (brussel sprouts) -symptomless.

Transmission

Transmitted by a vector; an insect; Brevicoryne brassicae (Tomlinson, Webb and Faithfull, 1972); Aphididae. Virus transmitted by mechanical inoculation; not transmitted by seed.

Geographical distribution

Spreads in Australia and the UK.

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show chlorosis, necrosis, sometimes symptomless.

Diagnostically susceptible host species and symptoms

• Brassica oleracea - vein-clearing and leaf-rolling, later symptomless.
• Datura stramonium - chlorotic and necrotic local lesions, then systemic mosaic and necrosis of veins.
• Nicotiana glutinosa -sometimes local lesions, then systemic mosaic.

Maintenance and propagation hosts

Datura stramonium.

Assay hosts (Local lesions or Whole plants)

Datura stramonium (L/W).

Susceptible host species

• Brassica oleracea
• Brassica oleracea var. botrytis
• Brassica oleracea var. gemmifera
• Datura stramonium
• Nicotiana glutinosa

Families containing susceptible hosts

• Cruciferae (3/3)
• Solanaceae (2/2)

Physical and biochemical properties

Properties of particles in sap

TIP: 50 °C. LIV: 0.5-0.8 days (12-18 hours) at 23ºC), or 2 days (at 4ºC). DEP: log₁₀ minus 3-4. Infectivity of sap decreased by treatment with di-ethyl ether (Lin and Campbell, 1972). Electron microscopy: preparations stained with PTA-K have bullet-shaped virions.

Purification method

Lin and Campbell (1972).

Particle morphology

Virions rhabdo- or bullet-shaped; enveloped; with a clear modal length; of 266 nm (in PTA-K), or 275 nm (in UA); 66 nm wide (in PTA-K), or 75 nm wide (in UA). Basic helix obvious; pitch of basic helix 4.5 nm.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 874 S (»41). Density 1.183-1.195 g/cm³ in potassium tartrate (Lin and Campbell, 1972).

Biochemical properties

Genome consists of RNA; single-stranded; linear.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm (in membrane-bounded sacs; Hill and Campbell (1968)). Inclusions absent from infected cells. Other cellular changes: mitochondria swollen with few cristae (Hill and Campbell, 1968).

Taxonomy and relationships

Cytorhabdovirus: Rhabdoviridae

Virus(es) with serologically unrelated virions

Lettuce necrotic yellows and barley yellow striate mosaic viruses.

Additional comments on relationships

Virions of broccoli necrotic yellows and lettuce necrotic yellows viruses are similar in size and intracellular location but these viruses differ in host range, host reaction, geographical distribution and are serologically distinct.

Comments and References

References

• Campbell, R.N. and Lin, M.T. (1972). CMI/AAB Descr. Pl. Viruses No. 85, 4 pp.
• Garret, R.G. and O'Loughlin, G.T. (1976). Virology 76: 653.
• Hills, G.J. and Campbell, R.N. (1968). J. Ultrastruct. Res. 24: 134.
• Lin, M.T. and Campbell, R.N. (1972). Virology 48: 30.
• Tomlinson, J.A., Webb, M.J.W. and Faithfull, E.M. (1972). Ann. appl. Biol. 71: 127.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.