Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Broad bean true mosaic comovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by A.J. Gibbs, 1981. Revised 1987.

Nomenclature

Synonyms

Vicia virus 1, Echtes Ackerbohnenmosaikvirus.

Acronym

BBTMV

ICTV decimal code

18.0.1.0.006

Host range and symptoms

Natural host range and symptoms

Symptoms vary cyclically over a few weeks.
• Vicia faba - mild mosaic and malformation, necrosis.

Transmission

Transmitted by a vector; an insect; Apion vorax, Sitonia spp. and other weevils; Coleoptera. Virus transmitted by mechanical inoculation; transmitted by seed.

Geographical distribution

Spreads in the African region and the Eurasian region; China, Morocco, and the UK. Found, but with no evidence of spread, in Australia in crops grown from imported seed.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Pisum sativum - systemic chlorosis, shoot necrosis.
• Vicia faba - mottle, mosaic and shoot necrosis.

Diagnostically insusceptible host species

Chenopodium amaranticolor, Nicotiana clevelandii.

Maintenance and propagation hosts

Pisum sativum, Vicia faba.

Assay hosts (Local lesions or Whole plants)

Pisum sativum (W), Vicia faba (W).

Susceptible host species

• Cicer arietinum
• Lens culinaris
• Lupinus albus
• Melilotus albus
• Phaseolus vulgaris
• Pisum sativum
• Trifolium incarnatum
• Trifolium repens
• Vicia faba
• Vicia sativa
• Vicia villosa

Insusceptible host species

• Antirrhinum majus
• Chenopodium amaranticolor
• Cucumis sativus
• Datura stramonium
• Glycine max
• Lathyrus odoratus
• Lotus corniculatus
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana tabacum
• Phaseolus lunatus
• Spinacia oleracea
• Stellaria media
• Trifolium hybridum
• Trifolium pratense

Families containing susceptible hosts

• Leguminosae-Papilionoideae (11/17)

Families containing insusceptible hosts

• Caryophyllaceae (1/1)
• Chenopodiaceae (2/2)
• Cucurbitaceae (1/1)
• Leguminosae-Papilionoideae (6/17)
• Scrophulariaceae (1/1)
• Solanaceae (4/4)

Sources of host-range data

Quantz (1953).

Physical and biochemical properties

Properties of particles in sap

TIP: 65-75 °C. LIV: 6-7 days. DEP: log₁₀ minus 4. Infectivity of sap not changed by treatment with di-ethyl ether. Leaf sap contains many virions.

Purification method

Blevings and Stace-Smith (1976).

Particle morphology

Virions isometric; not enveloped; 28 nm in diameter; angular in profile; without a conspicuous capsomere arrangement.

Physical properties

Three sedimenting components in purified preparations (in reducing buffer); sedimentation coefficient of the fastest 116 S (B); of the other(s) 95 S (M), or 59 S (T). A₂₆₀/A₂₈₀ ratio 1.81 (B), or 1.76 (M).

Biochemical properties

Virions contain 35 % nucleic acid (B), or 26 % nucleic acid (M), or 0 % nucleic acid (T); 65 % protein (B), or 74 % protein (M), or 100 % protein (T).

Genome consists of RNA; single-stranded; linear. Base composition 22.8 % G; 26.5 % A; 18.4 % C; 32.3 % U. Infectivity retained when deproteinised with phenol or detergent.

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

Virion protein(s) two; M_r of the larger 37500. M_r of 2nd largest 24500. Method of preparation: Blevings and Stace-Smith (1976).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm. Inclusions present in infected cells; are unusual in shape; are tubules from plasmodesmata containing rows of virus virions, also vesiculated bodies and virus aggregates (Russo et al., 1982).

Taxonomy and relationships

Comovirus: Comoviridae

Virus(es) with serologically related virions

Broad bean stain, glycine mosaic, radish mosaic and squash mosaic viruses.

Comments and References

References

• Blevings, S. and Stace-Smith, R. (1976). J. gen. Virol. 31: 199.
• Cockbain, A.J., Cook, S.M. and Bowen, R. (1975). Ann. appl. Biol. 81: 331.
• Cockbain, A.J., Bowen, R. and Vorra-Urai, S. (1976). Ann. appl. Biol. 84: 321.
• Gibbs, A.J. and Paul, H.L. (1970). CMI/AAB Descr. Pl. Viruses No. 20, 4 pp.
• Gibbs, A.J., Giussani-Belli, G. and Smith, H.G. (1968). Ann. appl. Biol. 61: 99.
• Jones, A.T. (1978). Ann. appl. Biol. 88: 137.
• Quantz, L. (1953). Phytopath. Z. 20: 421.
• Randles, J.W. and Dube, A.J. (1977). Australas. Pl. Path. Soc. Newsl. 6: 37.
• Russo, M., Castellano, M.A. and Martelli, G.P. (1982). J. Submicrosc. Cytol. 14: 149.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.