Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Broad bean stain comovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by A.J. Gibbs, 1981. Revised 1987.

Nomenclature

Synonyms

virus de la mosaique de la fève, MF virus, broad bean F1 virus (Gibbs et al., 1968), broad bean Evesham stain virus.

Acronym

BBSV

Strains

pea green mottle virus.

ICTV decimal code

18.0.1.0.005

Host range and symptoms

Natural host range and symptoms

Symptoms vary cyclically over a few weeks, or vary seasonally.
• Vicia faba - systemic mottling, necrosis and malformation.

Transmission

Transmitted by a vector; an insect; Apion vorax, Sitona spp.; Coleoptera. Virus transmitted by mechanical inoculation; transmitted by grafting; transmitted by seed (up to 10% in some Vicia faba cultivars).

Geographical distribution

Spreads in the African region and the Eurasian region. Found, but with no evidence of spread, in Australia from imported seed.

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show mottle, mosaic, necrosis.

Diagnostically susceptible host species and symptoms

• Phaseolus vulgaris cv. Tendergreen - systemic mottle.
• Pisum sativum, Vicia faba - systemic mottle; necrosis.

Diagnostically insusceptible host species

Chenopodium amaranticolor, Phaseolus vulgaris cv. Pinto, Nicotiana clevelandii.

Maintenance and propagation hosts

Phaseolus vulgaris cv. Tendergreen, Pisum sativum, Vicia faba.

Assay hosts (Local lesions or Whole plants)

Phaseolus vulgaris cv. Tendergreen (L), Vicia faba (W).

Susceptible host species

• Chenopodium album
• Chenopodium capitatum
• Chenopodium murale
• Chenopodium quinoa
• Cicer arietinum
• Glycine max
• Lathyrus odoratus
• Melilotus albus
• Nicotiana debneyi
• Phaseolus vulgaris
• Pisum sativum
• Trifolium hybridum
• Trifolium incarnatum
• Vicia faba
• Vigna unguiculata

Insusceptible host species

• Cassia occidentalis
• Chenopodium amaranticolor
• Cyamopsis tetragonoloba
• Datura stramonium
• Gomphrena globosa
• Lens culinaris
• Lotus corniculatus
• Medicago sativa
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana megalosiphon
• Nicotiana rustica
• Nicotiana sylvestris
• Nicotiana tabacum
• Phaseolus vulgaris (cv. Pinto)
• Trifolium pratense
• Trifolium repens
• Vigna radiata

Families containing susceptible hosts

• Chenopodiaceae (4/5)
• Leguminosae-Papilionoideae (10/17)
• Solanaceae (1/8)

Families containing insusceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (1/5)
• Leguminosae-Caesalpinioideae (1/1)
• Leguminosae-Papilionoideae (8/17)
• Solanaceae (7/8)

Sources of host-range data

Thornberry (1966); Moghal and Francki (1974); Musil et al. (1978); Valenta et al. (1979).

Physical and biochemical properties

Properties of particles in sap

TIP: 60-65 °C. LIV: 31 days. DEP: log₁₀ minus 3. Infectivity of sap not changed by treatment with di-ethyl ether. Leaf sap contains many virions.

Purification method

Gibbs et al. (1968).

Particle morphology

Virions isometric; not enveloped; 28 nm in diameter; angular in profile; without a conspicuous capsomere arrangement.

Physical properties

Three sedimenting components in purified preparations; sedimentation coefficient of the fastest 127 S (B); of the other(s) 100 S (M), or 60 S (T).

Biochemical properties

Virions contain 34 % nucleic acid (B), or 25 % nucleic acid (M), or 0 % nucleic acid (T); 66 % protein (T), or 75 % protein (M), or 100 % protein (T); 0 % lipid.

Genome consists of RNA; single-stranded. Total genome size 11.25 kb. Genome of two parts; largest (or only) genome part the larger 6.75 kb; the 2nd largest 4.5 kb. Base composition 23.3 % G; 26.5 % A; 18.4 % C; 31.9 % U. Infectivity retained when deproteinised with phenol or detergent.

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm. Inclusions present in infected cells; are unusual in shape; rows of virions in tubules, finger-like outgrowths at plasmodesmata, vacuolate-vesiculate bodies and virus aggregates (Russo et al., 1982).

Taxonomy and relationships

Comovirus: Comoviridae

Virus(es) with serologically related virions

Cowpea mosaic, cowpea severe mosaic, glycine mosaic, red clover mottle and squash mosaic viruses, but distantly (Beczner et al., 1983).

Comments and References

References

• Beczner, L., Musil, M. and Kowalska, C. (1983). Zesz. probl. Poste. Nauk rolm. 291: 21.
• Cockbain, A.J., Cook, S.M. and Bowen, R.(1975). Ann. appl. Biol. 81: 331.
• Gibbs, A.J. and Smith, H.G. (1970). CMI/AAB Descr. Pl. Viruses No. 29, 3 pp.
• Gibbs, A.J., Giussani-Belli, G. and Smith, H.G. (1968). Ann. appl. Biol. 61: 99.
• Jones, A.T. and Barker, H. (1976). Ann. appl. Biol. 83: 231.
• Lloyd, A.T.E., Smith, H.G. and Jones, L.H. (1965). Hort. Res. 5: 13.
• Moghal, S.M. and Francki, R.I.B. (1974). Aust. J. biol. Sci. 27: 341.
• Musil, M., Leskova, O. and Kleja, S. (1978). Ochr. Rost. 14, 161.
• Russo, M., Castellano, M.A. and Martelli, G.P. (1982). J. Submicrosc. Cytol. 14: 149.
• Valenta, V., Gressnerova, M., Marcinka, K. and Nermut, M.V. (1979). Acta Virol., Prague 13: 422.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.