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Descriptions and Lists from the VIDE Database

Broad bean mottle bromovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by A.J. Gibbs, 1981, Revised 1985.

Nomenclature

Acronym

BBMV

ICTV decimal code

10.0.3.0.002

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Vicia faba - leaf mottling and malformation.

Transmission

Transmitted by a vector; an insect; Acalymma trivittata, Colaspis flavida, Diabrotica undecimpunctata, Sitona lineata; Coleoptera. Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by seed.

Geographical distribution

Spreads in Portugal, Sudan, and the UK.

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show local lesions, mosaics, mottles.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor - chlorotic local lesions; not systemic.
• Cyamopsis tetragonoloba - necrotic local lesions or streaks.
• Phaseolus vulgaris cv. Canadian Wonder - chlorotic local lesions; systemic mottle and vein yellowing or netting.
• Pisum sativum - lethal systemic wilt.
• Vicia faba - systemic blotchy mottle.
• Nicotiana clevelandii - mild mottle.

Diagnostically insusceptible host species

Nicotiana tabacum.

Maintenance and propagation hosts

Vicia faba, Nicotiana clevelandii.

Assay hosts (Local lesions or Whole plants)

Cyamopsis tetragonoloba (L).

Susceptible host species

• Chenopodium album
• Chenopodium amaranticolor
• Chenopodium hybridum
• Chenopodium quinoa
• Cyamopsis tetragonoloba
• Glycine max
• Gomphrena globosa
• Lathyrus odoratus
• Lupinus albus
• Medicago sativa
• Melilotus albus
• Nicotiana clevelandii
• Phaseolus vulgaris
• Pisum sativum
• Trifolium hybridum
• Trifolium incarnatum
• Trifolium pratense
• Trifolium repens
• Trifolium subterraneum
• Vicia faba
• Vicia villosa

Insusceptible host species

• Cucumis sativus
• Datura stramonium
• Lablab purpureus
• Lycopersicon pimpinellifolium
• Nicotiana glutinosa
• Nicotiana tabacum
• Spinacia oleracea
• Vicia sativa
• Vigna radiata
• Vigna unguiculata

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (4/5)
• Leguminosae-Papilionoideae (15/19)
• Solanaceae (1/5)

Families containing insusceptible hosts

• Chenopodiaceae (1/5)
• Cucurbitaceae (1/1)
• Leguminosae-Papilionoideae (4/19)
• Solanaceae (4/5)

Sources of host-range data

Thornberry (1966); Lane (1974; 1981).

Physical and biochemical properties

Properties of particles in sap

TIP: 95 °C. LIV: 20 days. DEP: log₁₀ minus 3. Infectivity of sap not changed by treatment with di-ethyl ether. Leaf sap contains many virions.

Purification method

Lane (1974).

Particle morphology

Virions isometric; not enveloped; 26 nm in diameter; rounded in profile; without a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 85 S (pH 7). Density 1.36 g cm^-3 in CsCl (pH 6 or lower). Isoelectric point pH 5.6 (and 0.1 ionic strength). A₂₆₀/A₂₈₀ ratio 5.4.

Biochemical properties

Virions contain 21-23 % nucleic acid; 77-79 % protein; 0 % lipid. Also spermidine.

Genome consists of RNA; single-stranded; linear. Total genome size 8.25 kb. Genome of three parts; largest (or only) genome part the largest 3.158 kb; the 2nd largest 2.799 kb; the 3rd largest 2.293 kb. Base composition 25 % G; 27 % A; 19 % C; 29 % U. 5´ terminus of RNA has a methylated nucleotide cap. Infectivity retained when deproteinised with phenol or detergent. Genome has tRNA-like activity. Genome accepts tyrosine.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• K01776 Em(40)_vi:BRRNA1 Gb(84)_vi:BBMRNA1 Broad bean mottle virus RNA 1, 3´ end. 6/94 200bp.
• K01777 Em(40)_vi:BRRNA2 Gb(84)_vi:BBMRNA2 Broad bean mottle virus rna 2, 3´ end. 6/94 214bp.
• K01778 Em(40)_vi:BRRNA3 Gb(84)_vi:BBMRNA3 Broad bean mottle virus rna 3/rna 4, 3´ end. 6/94 266bp.
• M60291 Em(40)_vi:BBM3ACT Gb(84)_vi:BBM3ACT Broad bean mottle virus 3a and coat proteins, complete cds. 4/94 2,293bp.
• M64713 Em(40)_vi:BBMRNA2Q Gb(84)_vi:BBMRNA2Q Broad bean mottle virus RNA 2, complete cds. 5/91 2,799bp.
• M65138 Em(40)_vi:BBMIAP Gb(84)_vi:BBMIAP Broad bean mottle virus (BBMV) 1a protein gene, complete cds. 8/92 3,158bp.
• U24495 Em(43)_vi:Bb24495 Gb(89)_vi:Bbu24495 Broad bean mottle virus Type strain 2a protein gene, complete cds. 5/95 2,834bp.
• U24496 Em(43)_vi:Bb24496 Gb(89)_vi:Bbu24496 Broad bean mottle virus Mo strain 2a protein gene, complete cds. 5/95 2,917bp.

Features of the genome

Non-genomic nucleic acid found in the virions. Sub-genomic mRNA found in infected cells.

Features of proteins

Virion protein(s) one; M_r 20000. Method of preparation: Gibbs (1972): 1M NaCl and reducing agent.

Replication

Coat protein mRNA translated in the cytoplasm (cycloheximide sensitive). Replication does not depend on a helper virus.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm. Inclusions present in infected cells; are amorphous X-bodies; they contain virions.

Taxonomy and relationships

Bromovirus: Bromoviridae

Virus(es) with serologically related virions

Brome mosaic and cowpea chlorotic mottle viruses.

Comments and References

References

• Bawden, F.C., Chaudhuri, R.P. and Kassanis, B. (1951). Ann. appl. Biol. 38: 774.
• Dzianott, A.M. and Bujarski, J.J. (1991). Virology 185: 553.
• Gibbs, A.J. (1972). CMI/AAB Descr. Pl. Viruses No. 101, 4 pp.
• Lane, L.C. (1974). Adv. Virus Res. 19: 151.
• Lane, L.C. (1979). CMI/AAB Descr. Pl. Viruses No. 215, 4 pp.
• Lane, L.C. (1981). In: Handbook of Pl. Virus Infections and Comparative Diagnosis, p.333; ed. E. Kurstak. Elsevier/North Holland Biomedical Press, Amsterdam.
• Thornberry, H.H. (1966). In: Index of Plant Virus Diseases. U.S. Dep. Agric. Hdbk No. 307, p.235.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.