Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Blueberry scorch carlavirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by A.A. Brunt and R.R. Martin, 1991.

Nomenclature

Acronym

BlSV

Strains

Sheep Pen Hill virus.

ICTV decimal code

14.0.1.0.003

Host range and symptoms

Natural host range and symptoms

Symptoms persist. Symptoms flower necrosis and leaf chlorosis and necrosis.
• Vaccinium corymbosum - marginal necrosis or chlorosis of leaves, flowers aborted; some cultivars remain symptomless.

Transmission

Virus not transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants.

Geographical distribution

Spreads in the USA (Oregon and Washington).

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Vaccinium corymbosum - marginal leaf chlorosis.

Maintenance and propagation hosts

Vaccinium corymbosum.

Assay hosts (Local lesions or Whole plants)

Vaccinium corymbosum cv. Pemberton (W).

Susceptible host species

• Chenopodium quinoa
• Vaccinium corymbosum

Insusceptible host species

• Beta vulgaris
• Brassica campestris ssp. rapa
• Brassica juncea
• Catharanthus roseus
• Chenopodium amaranticolor
• Chenopodium murale
• Cucumis sativus
• Datura stramonium
• Gomphrena globosa
• Lactuca sativa
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana rustica
• Nicotiana sylvestris
• Nicotiana tabacum
• Petunia × hybrida
• Phaseolus vulgaris
• Pisum sativum
• Trifolium incarnatum
• Trifolium repens
• Verbesina encelioides
• Vigna unguiculata
• Zinnia elegans

Families containing susceptible hosts

• Chenopodiaceae (1/4)
• Ericaceae (1/1)

Families containing insusceptible hosts

• Amaranthaceae (1/1)
• Apocynaceae (1/1)
• Chenopodiaceae (3/4)
• Compositae (3/3)
• Cruciferae (2/2)
• Cucurbitaceae (1/1)
• Leguminosae-Papilionoideae (5/5)
• Solanaceae (8/8)

Comments on host-range

Only blueberry infected by most strains, one infects Chenopodium quinoa.

Sources of host-range data

Martin and Bristow (1988).

Physical and biochemical properties

Properties of particles in sap

Leaf sap contains few virions.

Purification method

Martin and Bristow (1988).

Particle morphology

Virions filamentous; not enveloped; usually straight; with a clear modal length; of 690 nm; 14 nm wide. Axial canal obscure. Basic helix obscure.

Physical properties

One sedimenting component in purified preparations. Density 1.28 g cm^-3 in CsCl. A₂₆₀/A₂₈₀ ratio 1.2.

Biochemical properties

Genome consists of RNA; single-stranded; linear. Total genome size 8.4 kb. Genome unipartite; largest (or only) genome part 8.4 kb. Genomic nucleic acid isolated by Martin and Bristow (1988).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• L25658 Em(40)_vi:BLUORF Gb(84)_vi:BLUORF Blueberry scorch carlavirus mRNA. 4/94 8,512bp. 1 sequence.

Features of proteins

Virion protein(s) one; M_r 35200; coat protein. Method of preparation: Martin and Bristow (1988).

Cytopathology

Virions found in leaves, roots, mesophyll and epidermis; in cytoplasm. Inclusions present in infected cells; are viroplasms; they contain virions.

Taxonomy and relationships

Carlavirus

Virus(es) with serologically related virions

Carnation latent, chrysanthemum B, dandelion, elderberry, helenium S, potato M, potato S, pea streak and red clover vein mosaic carlaviruses.

Best tests for diagnosis

Serological tests, ELISA is best. Due to the low pH of blueberry leaf sap, it is necessary to ensure the pH of homogenized leaves is above pH 6.0 by using a strong buffer (10 × PBS or 0.5% nicotine).

Comments and References

References

• MacDonald, S.G., Martin, R.R. and Bristow, P.R. (1989). Acta Hort. 241: 295.
• Martin, R.R. and Bristow, P.R. (1988). Phytopathology 78: 1636.
• Martin, R.R. and MacDonald, S.G. (1988). Can. Pl. Dis. Surv. 68: 98.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.