Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Blueberry leaf mottle nepovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by D.C. Ramsdell, 1987.

Nomenclature

Acronym

BLMV

Strains

blueberry leaf mottle virus - M1 and blueberry leaf mottle virus - NY.

ICTV decimal code

18.0.3.0.006

Host range and symptoms

Natural host range and symptoms

• Vaccinium corymbosum cv. Rubel - stem dieback and poor regrowth, hence stunting. Leaves malformed and mottled.
• V. corymbosum cv. Jersey - stem dieback. Regrowth stunted. Leaves rosetted and mottled.

Transmission

Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; transmitted by pollen to the pollinated plant (by honeybees).

Ecology and control

Studies reported by Childress and Ramsdell (1986a); Childress and Ramsdell (1986b); Childress and Ramsdell (1987).

Geographical distribution

Spreads in the USA (Michigan). Found, but with no evidence of spread, in New York, USA (grapevine) and in Pleven, Bulgaria.

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show local lesions, mottle, tip necrosis.

Diagnostically susceptible host species and symptoms

• Chenopodium quinoa, C. album - chlorotic local lesions, systemic mottle and tip necrosis.
• C. amaranticolor - systemic mottle.
• Nicotiana clevelandii - systemic necrosis.

Maintenance and propagation hosts

Chenopodium amaranticolor, C. quinoa respectively.

Assay hosts (Local lesions or Whole plants)

Chenopodium quinoa (L), C. amaranticolor (W), Nicotiana clevelandii (W).

Susceptible host species

• Chenopodium album
• Chenopodium amaranticolor
• Chenopodium capitatum
• Chenopodium foetidum
• Chenopodium murale
• Chenopodium quinoa
• Cucumis sativus
• Cyamopsis tetragonoloba
• Gomphrena globosa
• Lycopersicon esculentum
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana debneyi
• Nicotiana glutinosa
• Nicotiana rustica
• Nicotiana sylvestris
• Nicotiana tabacum
• Phaseolus vulgaris
• Vaccinium corymbosum

Insusceptible host species

• Cassia occidentalis
• Catharanthus roseus
• Cucurbita maxima
• Cucurbita pepo
• Datura stramonium
• Momordica balsamina
• Petunia × hybrida
• Plantago lanceolata
• Plantago major
• Sesbania exaltata
• Tithonia speciosa
• Vigna unguiculata
• Vigna unguiculata ssp. cylindrica
• Zinnia elegans

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (6/6)
• Cucurbitaceae (1/4)
• Ericaceae (1/1)
• Leguminosae-Papilionoideae (2/5)
• Solanaceae (8/10)

Families containing insusceptible hosts

• Apocynaceae (1/1)
• Compositae (2/2)
• Cucurbitaceae (3/4)
• Leguminosae-Caesalpinioideae (1/1)
• Leguminosae-Papilionoideae (3/5)
• Plantaginaceae (2/2)
• Solanaceae (2/10)

Sources of host-range data

Ramsdell and Stace-Smith (1979).

Physical and biochemical properties

Properties of particles in sap

TIP: 65-70 °C. LIV: 14-21 days (at 4ºC), or 5-7 days (at 20ºC). DEP: log₁₀ minus 4-5. Leaf sap contains few virions.

Purification method

Ramsdell and Stace-Smith (1983).

Particle morphology

Virions isometric; not enveloped; 28 nm in diameter; angular in profile; without a conspicuous capsomere arrangement.

Physical properties

Three sedimenting components in purified preparations; sedimentation coefficient of the fastest 128 S (B); of the other(s) 120 S (M), or 53 S (T). Density 1.497 g cm^-3 in CsCl (B), or 1.471 g cm^-3 in CsCl (M). Isoelectric point pH 7.5.

Biochemical properties

Genome consists of RNA; single-stranded; linear. Total genome size 14.6 kb. Genome of two parts; largest (or only) genome part the larger 7.6 kb; the 2nd largest 7 kb. Genomic nucleic acid isolated by Ramsdell and Stace-Smith (1981).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• U20621 Em(43)_vi:Bl20621 Gb(89)_vi:Blu20621 Blueberry leaf mottle virus segment RNA2 polyprotein gene, partial cds. 5/95 3,082bp.
• U20622 Em(43)_vi:Bl20622 Gb(89)_vi:Blu20622 Blueberry leaf mottle virus segment RNA1 polyprotein gene, partial cds. 5/95 1,908bp.

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

M_r 54000; coat protein. Method of preparation: Ramsdell and Stace-Smith (1981).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves, roots and blossoms; in cytoplasm; the cytoplasm of parenchyma cells close to endoplasmic reticulum but not with other organelles. Inclusions absent from infected cells.

Taxonomy and relationships

Nepovirus: Comoviridae

Virus(es) with serologically related virions

Grapevine Bulgarian latent virus is distantly related.

Comments and References

References

• Childress, A.M. and Ramsdell, D.C. (1986a). Acta Hort. 186: 87.
• Childress, A.M. and Ramsdell, D.C. (1986b). Phytopathology 76: 1333.
• Childress, A.M. and Ramsdell, D.C. (1987). Phytopathology 77: 167.
• Ramsdell, D.C. (1987). In: Virus Diseases of Small Fruits; ed. R.H. Converse. U.S. Dep. Agric. Hdbk No. 631, p. 112.
• Ramsdell, D.C. and Stace-Smith, R. (1979). Acta Hort. 95: 37.
• Ramsdell, D.C. and Stace-Smith, R. (1980). Proc. 7th Meeting Int. Counc. for the Study of Viruses and Virus-Like Dis. of Grapevine, Niagara Falls, Canada.
• Ramsdell, D.C. and Stace-Smith, R. (1981). Phytopathology 71: 468.
• Ramsdell, D.C. and Stace-Smith, R. (1983). CMI/AAB Descr. Pl. Viruses No. 267, 4 pp.
• Uyemoto, J.K., Taschenberg, E.F. and Hummer, D.K. (1977). Pl. Dis. Reptr 61: 949.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.