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Descriptions and Lists from the VIDE Database

Belladonna mottle tymovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by A.J. Gibbs, 1991.

Nomenclature

Synonyms

Tollkirschenscheckungs virus.

Acronym

BeMV

ICTV decimal code

77.0.1.0.002

Host range and symptoms

Natural host range and symptoms

Symptoms persist. Symptoms mottling.
• Atropa belladonna - mottling, distortion.

Transmission

Transmitted by a vector; an insect; Epithrix atropae (Jankulova et al., 1968); Coleoptera. Not transmitted by aphids. Virus transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed.

Geographical distribution

Spreads in Bulgaria and Germany.

Experimental host range

Few (<3) families susceptible. Experimentally infected plants mostly show necrosis, mosaics.

Diagnostically susceptible host species and symptoms

• Chenopodium quinoa - local lesions, not systemic.
• Datura stramonium - necrotic local lesions, mosaic.
• Nicotiana tabacum - ring local lesions, vein clearing, mosaic.

Maintenance and propagation hosts

Nicotiana tabacum.

Assay hosts (Local lesions or Whole plants)

Nicotiana tabacum (L).

Susceptible host species

• Atropa belladonna
• Catharanthus roseus
• Chenopodium quinoa
• Datura stramonium
• Gypsophila elegans
• Leptosiphon
• Nicotiana tabacum
• Sinningia speciosa

Insusceptible host species

• Antirrhinum majus
• Arachis hypogaea
• Bellis perennis
• Beta vulgaris
• Brassica campestris ssp. pekinensis
• Catalpa bignonioides
• Cucumis sativus
• Daucus carota
• Delphinium hybridum
• Eucalyptus cloeziana
• Euphorbia marginata
• Fagopyrum esculentum
• Fragaria vesca
• Gossypium hirsutum
• Helianthus annuus
• Ipomoea nil
• Lactuca sativa
• Lobelia erinus
• Papaver nudicaule
• Pastinaca sativa
• Petroselinum crispum
• Pisum sativum
• Podolepis robusta
• Salvia splendens
• Tropaeolum majus
• Valeriana officinalis
• Vigna radiata
• Viola cornuta

Families containing susceptible hosts

• Apocynaceae (1/1)
• Caryophyllaceae (1/1)
• Chenopodiaceae (1/2)
• Gesneriaceae (1/1)
• Polemoniaceae (1/1)
• Solanaceae (3/3)

Families containing insusceptible hosts

• Bignoniaceae (1/1)
• Campanulaceae (1/1)
• Chenopodiaceae (1/2)
• Compositae (4/4)
• Convolvulaceae (1/1)
• Cruciferae (1/1)
• Cucurbitaceae (1/1)
• Euphorbiaceae (1/1)
• Labiatae (1/1)
• Leguminosae-Papilionoideae (3 /3)
• Malvaceae (1/1)
• Myrtaceae (1/1)
• Papaveraceae (1/1)
• Polygonaceae (1/1)
• Ranunculaceae (1/1)
• Rosaceae (1/1)
• Scrophulariaceae (1/1)
• Tropaeolaceae (1/1)
• Umbelliferae (3 /3)
• Valerianaceae (1/1)
• Violaceae (1/1)

Sources of host-range data

Paul et al. (1968); Guy et al. (1984).

Physical and biochemical properties

Properties of particles in sap

TIP: 80 °C. LIV: 5-20 days. DEP: log₁₀ minus 6-7. Infectivity of sap not changed by treatment with di-ethyl ether. Leaf sap contains many virions.

Purification method

Steere's method.

Particle morphology

Virions isometric; not enveloped; 27 nm in diameter; rounded in profile; with a conspicuous capsomere arrangement.

Physical properties

Two sedimenting components in purified preparations; sedimentation coefficient of the fastest 113 S; of the other(s) 53 S. Isoelectric point pH 9.12.

Biochemical properties

Virions contain 37 % nucleic acid; 63 % protein.

Genome consists of RNA; single-stranded; linear. Total genome size 6.3 kb. Genome unipartite; largest (or only) genome part 6.3 kb. Base composition 17.5 % G; 22.8 % A; 32.8 % C; 26.9 % U. 5´ terminus of RNA has a methylated nucleotide cap. Infectivity retained when deproteinised with proteases; retained when deproteinised with phenol or detergent. Poly A region absent. Additional factor not required for infectivity. Genome has tRNA-like activity. Nucleotide sequence references: Ding et al. (1990): EMBL X54529.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• X54529 Em(40)_vi:BMVVP Gb(84)_vi:BMVVP Belladonna mottle virus VP gene for virion protein and ORF. 9/93 615bp.

Features of the genome

Features of the genome: Untranslated 5´ terminal region, overlapping gene of about 1800 nucleotides followed 7 nucleotides (to 3´) by replicase gene of about 5400 nucleotides, then after the conserved tymobox promoter region by the virion protein gene of 567 nucleotides.

Sub-genomic mRNA found in infected cells; mRNA of virion protein.

Features of proteins

Virion protein(s) one; M_r 19784; coat protein. Amino acid sequence: Ding et al. (1990). Virion proteins not glycosylated. Virion proteins not phosphorylated.

Replication

Genome replicates in association with chloroplasts (in vesicles in the chloroplast external membrane). Coat protein mRNA translated in the cytoplasm. Replication does not depend on a helper virus.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm. Inclusions absent from infected cells.

Taxonomy and relationships

Tymovirus

Virus(es) with serologically related virions

Andean potato latent, dulcamara mottle, eggplant mosaic, ononis yellow mosaic and scrophularia mottle viruses.

Comments and References

References

• Bode, O. and Marcus, O. (1959). Proc. 4th Int. Cong. Crop Protect. Hamburg 1: 375.
• Ding, S.W., Howe, J., MacKenzie, A., Skotnicki, M. and Gibbs, A.J. (1990). Nucl. Acids Res. 18: 6138.
• Guy, P.L., Dale, J.L., Adena, M.A. and Gibbs, A.J. (1984). Pl. Path. 33: 337.
• Koenig, R. (1976). Virology 72: ;1.
• Paul, H.L. (1971). CMI/AAB Descr. Pl. Viruses No. 52, 3 pp.
• Paul, H.L., Bode, O., Jankulova, M. and Brandes, J. (1968). Phytopath. Z. 61: 342.
• Peter, R., Peter, C., Dupin, A. and Witz, J. (1989). C.r. Acad. Sci., Paris 309: 599.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.