Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Bean leaf roll luteovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by J.W. Ashby, 1981. Revised 1989 by R.R. Martin and for chickpea stunt strain by Y.L. Nene, 1981 and revised 1983.

Nomenclature

Synonyms

alfalfa Michigan luteovirus (Thottappilly et al. 1977), legume yellows virus, pea leaf roll virus, pea tip yellowing virus, pea top yellows virus.

Acronym

BLRV

Strains

chickpea stunt virus.

ICTV decimal code

39.0.1.0.007

Host range and symptoms

Natural host range and symptoms

Symptoms persist, or disappear soon after infection.
• Medicago sativa - usually symptomless.
• Pisum sativum - chlorotic, leaves rolled downward.
• Trifolium repens - yellowed, rolled leaves.
• Vicia faba - chlorotic leaves and flowers abscissed.
• Phaseolus vulgaris, Cicer arietinum, Vigna unguiculata, Lens culinaris - leaves yellowed.

Transmission

Transmitted by a vector; an insect; Acyrthosiphon pisum, Myzus persicae, Macrosiphum euphorbiae, Megoura viciae, Aphis craccivora (chickpea stunt strain); Aphididae. Transmitted in a persistent manner. Virus retained when the vector moults; does not multiply in the vector; can help the vector transmission of another virus (bean yellow vein banding umbravirus); not transmitted by mechanical inoculation; transmitted by grafting; not transmitted by seed; not transmitted by pollen.

Geographical distribution

Spreads in Ethiopia, Germany, Iran, Lebanon, Pakistan, Sudan, Syria, Tunisia, Turkey, the UK, and the USA (California, the Midwest and Northwest).

Experimental host range

Few (<3) families susceptible. Experimentally infected plants mostly show chlorosis, leaf rolling.

Diagnostically susceptible host species and symptoms

• Medicago hispida - systemic chlorosis.
• Pisum sativum cv. Onyx - tip leaves chlorotic, leaves rolled (cv. Kelvedon Wonder is insusceptible).
• Trifolium subterraneum - red leaf margins.
• Vicia faba - interveinal chlorosis, leaves rolled upward.

Diagnostically insusceptible host species

Beta vulgaris, Capsella bursa-pastoris, Lactuca sativa, Pisum sativum cv. Kelvedon Wonder.

Maintenance and propagation hosts

Pisum sativum cvs Galaxy, Onyx, Eurofin, Vicia faba, Medicago sativa.

Assay hosts (Local lesions or Whole plants)

Pisum sativum cv. Onyx (W), Trifolium subterraneum cv. Bacchus Marsh (W).

Susceptible host species

• Arachis hypogaea
• Astragalus sinicus
• Cicer arietinum
• Glycine max
• Lathyrus odoratus
• Lens culinaris
• Lupinus cosentinii
• Medicago hispida
• Medicago sativa
• Phaseolus vulgaris
• Pisum sativum
• Trifolium dubium
• Trifolium hybridum
• Trifolium incarnatum
• Trifolium pratense
• Trifolium repens
• Trifolium subterraneum
• Vicia faba
• Vicia sativa
• Vigna unguiculata

Insusceptible host species

• Beta vulgaris
• Brassica campestris ssp. napus
• Capsella bursa-pastoris
• Glycine max
• Gomphrena globosa
• Lablab purpureus
• Lactuca sativa
• Lens culinaris
• Linum usitatissimum
• Lotus corniculatus
• Lupinus albus
• Melilotus albus
• Phaseolus vulgaris
• Pisum sativum (cv. Kelvedon Wonder)
• Senecio vulgaris
• Stellaria media
• Tetragonia tetragonioides
• Trifolium pratense
• Vicia faba
• Zinnia elegans

Families containing susceptible hosts

• Leguminosae-Papilionoideae (20/24)

Families containing insusceptible hosts

• Amaranthaceae (1/1)
• Caryophyllaceae (1/1)
• Chenopodiaceae (1/1)
• Compositae (3/3)
• Cruciferae (2/2)
• Leguminosae-Papilionoideae (10/24)
• Linaceae (1/1)
• Tetragoniaceae (1/1)

Sources of host-range data

Cockbain and Gibbs (1973), Johnstone et al., (1984).

Physical and biochemical properties

Properties of particles in sap

Leaf sap contains few virions.

Purification method

Takanami and Kubo (1979); Ashby and Huttinga (1979).

Particle morphology

Virions isometric; not enveloped; 27 nm in diameter; rounded in profile; without a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations. Density 1.32 in Cs₂SO₄.

Biochemical properties

Virions contain 37.8 % nucleic acid.

Genome consists of RNA; single-stranded; linear. Total genome size 6 kb. Genome unipartite; largest (or only) genome part 6 kb. Genomic nucleic acid isolated by R.R. Martin; use hot phenol. Poly A region absent.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• X53865 Em(40)_vi:LUBLRVCP Gb(84)_vi:LUBLRVCP Bean leaf roll virus gene for coat protein. 9/93 591bp.
• U15978 Em(43)_vi:Bl15978 Gb(89)_vi:Blu15978 Bean leaf roll virus coat protein mRNA, complete cds. 11/94 591bp.

Features of proteins

Virion protein(s) one; M_r 23000 (type strain), or 30000-35000 (chickpea stunt strain).

Cytopathology

Virions found in phloem parenchyma mainly in necrotic sieve-tube cells; in cytoplasm. Inclusions absent from infected cells. Other cellular changes: necrosis and collapse of phloem.

Taxonomy and relationships

Luteovirus

Virus(es) with serologically related virions

Soybean dwarf, beet western yellows, potato leafroll, carrot red leaf and barley yellow dwarf (RPV) viruses.

Differences between type strain and others

The chickpea stunt strain of the virus (Kaiser and Danesh, 1966) has been recorded in the same geographical areas as the type strain. Hosts recorded for the chickpea stunt strain but not for the type strain are: Chenopodium amaranticolor (I); Cucumis sativus (I); Cyamopsis tetragonoloba (I); Datura stramonium (I); Nicotiana glutinosa (I); Nicotiana tabacum (I); Phaseolus lunatus (S); Vicia villosa (S); Vigna radiata (I) (Anon., 1978/79).

Best tests for diagnosis

ELISA tests with MAbs very effective.

Comments and References

References

• Anon. (1978-79). Rep. Int. Crops Res. Inst. for Semi-Arid Tropics 1978-79: 30.
• Ashby, J.W. (1984). CMI/AAB Descr. Pl. Viruses No. 286, 5 pp.
• Ashby, J.W. and Huttinga, H. (1979). Neth. J. Pl. Path. 85: 113.
• Cockbain, A.J. and Gibbs, A.J. (1973). Ann. appl. Biol. 73: 177.
• D'Arcy, C.J., Martin, R.R. and Torrance, L. (1988). Phytopathology 78: 1537.
• Fukano and Yokoyama (1951). Kyushu Nogyo Kenkyu 10: 133.
• Inouye, T. (1971). Ber. Ohara Inst. Landw. Biol. 15: 69.
• Inouye, T. and Asatari (1968). Ann. Phytopath. Soc. Japan 34: 317.
• Johnstone, G.R., Ashby, J.W., Gibbs, A.J., Duffus, J.E., Thottapilly, G. and Fletcher, J.D. (1984). Neth J. Pl. Path. 90: 107.
• Kaiser, W.J. (1972). FAO Pl. Prot. Bull. 20: 127.
• Kaiser, W.J. and Danesh, D. (1966). Phytopathology 61: 453.
• Kaiser, W.J and Danesh, D.(1971). Phytopathology 61: 372.
• Quantz, L. and Volk, J. (1954). NachrBl. dtsch. PflSchutz., Braunschweig 6: 177.
• Takanami, Y. and Kubo, S. (1979). J. gen. Virol. 44: 153.
• Thottappilly, G., Kao, Y.J., Hooper, G.R. and Bath, J.E. (1977). Phytopathology 67: 1451.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.