Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Artichoke vein banding (?) nepovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by D. Gallitelli, 1987.

Nomenclature

Acronym

AVBV.

ICTV decimal code

18.0.3.T.002

Host range and symptoms

Natural host range and symptoms

Symptoms vary seasonally.
• Cynara scolymus cvs Bayrombasa, Sakiz, Mazzaferrata - vein chlorosis.

Transmission

Virus transmitted by mechanical inoculation.

Geographical distribution

Found, but with no evidence of spread, in Europe (Apulia, Italy.

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show local lesions, severe systemic mosaic.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor, C. quinoa - small chlorotic or necrotic local lesions, severe mosaic and tip necrosis.
• Phaseolus vulgaris - pinpoint necrotic local lesions, mosaic, malformation of trifoliate leaves.

Maintenance and propagation hosts

Chenopodium quinoa, Phaseolus vulgaris.

Assay hosts (Local lesions or Whole plants)

Ocimum basilicum (L), Chenopodium quinoa (W), Phaseolus vulgaris (W).

Susceptible host species

• Chenopodium amaranticolor
• Chenopodium ambrosioides
• Chenopodium murale
• Chenopodium quinoa
• Cucumis sativus
• Cucurbita pepo
• Cynara scolymus
• Gomphrena globosa
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana rustica
• Ocimum basilicum
• Petunia × hybrida
• Phaseolus vulgaris
• Vicia faba
• Vigna radiata
• Vigna unguiculata
• Zinnia elegans

Insusceptible host species

• Amaranthus caudatus
• Antirrhinum majus
• Apium graveolens
• Beta vulgaris
• Brassica campestris ssp. pekinensis
• Brassica oleracea var. botrytis
• Calendula officinalis
• Carthamus tinctorius
• Celosia argentea
• Celosia cristata
• Datura metel
• Datura stramonium
• Dianthus barbatus
• Helianthus annuus
• Lactuca sativa
• Lathyrus odoratus
• Lupinus albus
• Lycopersicon esculentum
• Nicotiana tabacum
• Physalis floridana
• Pisum sativum
• Saponaria vaccaria
• Sinapis alba
• Solanum melongena
• Spinacia oleracea
• Tetragonia tetragonioides
• Tropaeolum majus
• Vigna unguiculata ssp. cylindrica

Families containing susceptible hosts

• Amaranthaceae (1/4)
• Chenopodiaceae (4/6)
• Compositae (2/6)
• Cucurbitaceae (2/2)
• Labiatae (1/1)
• Leguminosae-Papilionoideae (4/8)
• Solanaceae (5/11)

Families containing insusceptible hosts

• Amaranthaceae (3/4)
• Caryophyllaceae (2/2)
• Chenopodiaceae (2/6)
• Compositae (4/6)
• Cruciferae (3/3)
• Leguminosae-Papilionoideae (4/8)
• Scrophulariaceae (1/1)
• Solanaceae (6/11)
• Tetragoniaceae (1 /1)
• Tropaeolaceae (1/1)
• Umbelliferae (1/1)

Sources of host-range data

Gallitelli et al. (1978).

Physical and biochemical properties

Properties of particles in sap

TIP: 55 °C. LIV: 3 days. DEP: log₁₀ minus 5. Leaf sap contains many virions.

Purification method

Homogenize infected leaves in 100 mM neutral phosphate buffer. Clarify with 10% Mg-activated bentonite. Differential centrifugation.

Particle morphology

Virions isometric; not enveloped; 30 nm in diameter; angular in profile; without a conspicuous capsomere arrangement.

Physical properties

Three sedimenting components in purified preparations; sedimentation coefficient of the fastest 124 S (B); of the other(s) 92 S (M), or 56 S (T). Density virions are unstable even after fixation with formaldehyde.

Biochemical properties

Virions contain 37 % nucleic acid (B), or 24 % nucleic acid (M), or 0 % nucleic acid (T); 63 % protein (B), or 76 % protein (M), or 100 % protein (T).

Genome consists of RNA; single-stranded; linear. Total genome size 12.3 kb. Genome of two parts; largest (or only) genome part the larger 7.8 kb; the 2nd largest 4.5 kb. Genomic nucleic acid isolated by : using phenol-SDS. Infectivity retained when deproteinised with phenol or detergent.

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

Virion protein(s) four; M_r of the largest 27000. M_r of 2nd largest 24000. M_r of 3rd largest 22000. M_r of 4th largest 14300. Method of preparation: Laemmli (1970); Chu and Francki (1979).

Cytopathology

Virions found in mesophyll; in cytoplasm. Inclusions present in infected cells; are membranous bodies and unusual in shape; vesicles containing finely stranded material resembling nucleic acid; they do not contain virions.

Taxonomy and relationships

Possible Nepovirus: Comoviridae

Virus(es) with serologically unrelated virions

Arabis mosaic, artichoke Italian latent, artichoke yellow ringspot, cherry leaf roll, cherry rasp leaf, chicory yellow mottle, cacao necrosis, grapevine Bulgarian latent, grapevine chrome mosaic, grapevine fanleaf, myrobalan latent ringspot, olive latent ringspot, peach rosette, raspberry ringspot, strawberry latent ringspot, tobacco ringspot and tomato ringspot.

Best tests for diagnosis

Use ISEM. In nature artichoke vein banding virus may be mixed with artichoke latent potyvirus which has filamentous virions or with artichoke mottled crinkle or artichoke Italian latent viruses which are serologically distinct.

Comments and References

References

• Chu, P.W.G. and Francki, R.I.B. (1979). Virology 93: 398.
• Gallitelli, D., Martelli, G.P. and Rana, G.L. (1984). CMI/AAB Descr. Pl. Viruses No. 285, 4 pp.
• Gallitelli, D., Rana, G.L. and Di Franco, A. (1978). Phytopathol. Medit. 17: 1.
• Laemmli, U.K. (1970). Nature, Lond. 277: 680.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.