Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Agropyron mosaic rymovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by W.G. Langenberg and N.L. Robertson, 1991.

Nomenclature

Synonyms

agropyron green mosaic virus, agropyron streak mosaic virus, agropyron yellow mosaic virus, couch grass streak mosaic virus.

Acronym

AgMV

Strains

PV75 severe isolate, PV101, mild isolate.

ICTV decimal code

57.0.2.0.002

Host range and symptoms

Natural host range and symptoms

Symptoms persist and vary seasonally.
• Elytrigia repens, Triticum aestivum, T. durum - light green/yellow mosaic; leaves may become chlorotic and plants stunted.

Transmission

Transmitted by a vector; a mite; Abacarus hystrix; Eriophyidae. Not transmitted by Aculus mckenziei, Eriophyes tulipae. Virus transmitted by mechanical inoculation; not transmitted by seed; not transmitted by pollen.

Ecology and control

Studies reported by Slykhuis (1962; 1969).

Geographical distribution

Spreads in the Eurasian region and the North American region; Canada, Finland, the USA.

Experimental host range

Few (<3) families susceptible. Experimentally infected plants mostly show chlorotic mottle and streaks.

Diagnostically susceptible host species and symptoms

• Elytrigia repens, Elytrigia intermedia, Lophopyrum elongatum, Secale cereale, Triticum aestivum, T. durum - light green/yellow mosaic in leaves; plants may die.

Diagnostically insusceptible host species

Avena sativa, Bromus inermis, Echinochloa crus-galli, Hordeum jubatum, Lolium perenne.

Maintenance and propagation hosts

Elytrigia repens, Triticum aestivum.

Assay hosts (Local lesions or Whole plants)

Triticum aestivum (W).

Susceptible host species

• Bromus secalinus
• Elytrigia intermedia
• Elytrigia repens
• Hordeum vulgare
• Lolium multiflorum
• Lophopyrum elongatum
• Poa pratensis
• Secale cereale
• Setaria viridis
• Triticum aestivum
• Triticum durum

Insusceptible host species

• Avena sativa
• Bromus inermis
• Dactylis glomerata
• Echinochloa crus-galli
• Hordeum jubatum
• Lolium perenne

Families containing susceptible hosts

• Gramineae (11/17)

Families containing insusceptible hosts

• Gramineae (6/17)

Sources of host-range data

Slykhuis (1961; 1973); Slykhuis and Bell (1966).

Physical and biochemical properties

Properties of particles in sap

TIP: 50 °C. LIV: 2-4 days (at 20ºC). DEP: log₁₀ minus 3. Leaf sap contains many virions.

Purification method

Staples and Brakke (1963); Slykhuis and Bell (1966).

Particle morphology

Virions filamentous; not enveloped; usually flexuous; with a clear modal length; of 700-760 nm; 15 nm wide. Axial canal obscure.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 165 S.

Biochemical properties

Genome consists of RNA; single-stranded; linear. Total genome size 8-10 kb. Genome unipartite; largest (or only) genome part 8-10 kb. Genomic nucleic acid isolated by Langenberg and Robertson: polyadenylated full-length RNA genomes were obtained from partially purified virions with proteinase K and SDS, phenol/chloroform extraction, ethanol precipitation, and oligo-d(T) cellulose chromatography. 5´ terminus of RNA has a VPg. Poly A region the 3´ end has a poly (A) tail.

NCBI sequence data (Entrez search)

Features of the genome

Non-genomic nucleic acid not found in the virions. Sub-genomic mRNA not found in infected cells.

Features of proteins

Virion protein(s) one; coat protein.

Virus-coded non-virion proteins isolated (Langenberg (1991)); one protein found. M_r 68000-72000; pinwheels.

Replication

Genome replicates presumably in cytoplasm. Coat protein mRNA translated in the cytoplasm. Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves, roots, mesophyll, epidermis and the meristems; in cytoplasm. Inclusions present in infected cells; are pinwheels; they do not contain virions.

Taxonomy and relationships

Rymovirus: Potyviridae

Virus(es) with serologically related virions

Hordeum mosaic and wheat streak mosaic viruses.

Virus(es) with serologically unrelated virions

Oat necrotic mottle and ryegrass mosaic viruses.

Additional comments on relationships

The 3´ ends of the genomes of agropyron mosaic and hordeum mosaic viruses are 73% homologous.

Best tests for diagnosis

Use ISEM and immunogold labelling.

Comments and References

References

• Langenberg, W.G. (1991). J. gen. Virol. 72: 493.
• McKinney, H.H. (1937). U.S. Dept Agric. Circular No. 442, 23 pp.
• Robertson, N.L., French, R. and Langenberg, W.G. (1990). Phytopathology 80: 1018.
• Slykhuis, J.T. (1961). Can. J. Pl. Sci. 41: 211.
• Slykhuis, J.T. (1962). Can. J. Bot. 40: 1439.
• Slykhuis, J.T. (1969). Phytopathology 59: 29.
• Slykhuis, J.T. (1973). CMI/AAB Descr. Pl. Viruses No. 118, 4 pp.
• Slykhuis, J.T. and Bell, W. (1966). Can. J. Bot. 44: 1191.
• Staples, R. and Brakke, M. (1963). Phytopathology 53: 969.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.